We narrowed to 2,194 results for: D10

1. TFORF2021

   Plasmid

   #142314

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NPAS4 (NPAS4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFP-Stim1-D76A

   Plasmid

   #186622

   Purpose

   Encodes Stim1 mutant D76A fused to YFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Stromal Interaction Molecule 1 (STIM1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D76A

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2966

   Plasmid

   #144442

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR0B1 (NPAS4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpRY dCas9 VPR

   Plasmid

   #188511

   Purpose

   Expresses FLAG tagged SpRY dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. eGFP-KIF5A-Rigor

   Plasmid

   #172202

   Purpose

   Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 235 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT131_GalL_cCDA1-NGBE3

   Plasmid

   #145095

   Purpose

   Expressing base editor cCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.13-d

   Plasmid

   #136297

   Purpose

   expresses ABE8.13-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.13-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-mTET1-N

    Plasmid

    #81056

    Purpose

    bacterial expression of N-terminus of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    N-terminus, amino acids 301-1366

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-BE3-PGK-Puro

    Plasmid

    #110842

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-hA3A-BE3-C101S

    Plasmid

    #113426

    Purpose

    Expresses hA3A-BE3-C101S in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-C101S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_C101S

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-hA3A-BE3-C106S

    Plasmid

    #113427

    Purpose

    Expresses hA3A-BE3-C106S in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-C106S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_C106S

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-BE3-P2A-Puro

    Plasmid

    #110838

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-sp-myc-hSema4D

    Plasmid

    #190654

    Purpose

    Expresses Human Sema4D with N terminal myc after signal peptide

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4D (SEMA4D Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUHD 10-3-human CDK11 p110B wt.-FLAG

    Plasmid

    #240138

    Purpose

    Express CDK11 p110B gene

    Depositor

    Vincent Kidd, Martine Roussel, Jill Lahti

    Article

    Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

    Insert

    CDK11 p110 (CDK11A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE, CMV

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. shRNA_circMTO1_2

    Plasmid

    #215233

    Purpose

    Supression of shcircMTO1(2,RI,3)_2 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circMTO1 shRNA 2 (MTO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. shRNA_MTO1_2

    Plasmid

    #215213

    Purpose

    Supression of shMTO1_2 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    MTO1 shRNA 2 (MTO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Plasmid

    #200793

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    1-914; Δ505-610

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. STIM1-2Strep

    Plasmid

    #210361

    Purpose

    Expresses STIM1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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