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We narrowed to 4,673 results for: pcr

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Showing: 1801 - 1820 of 4673 results

  1. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    54469-plasmid-map-sequence-id-114883

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  3. pLO104

    Plasmid
    #90224
    Purpose
    Complements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)
    Depositor
    Berl Oakley
    Publication
    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATET_00531
    Use
    Aspergillus
    Promoter
    pLac
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90224-plasmid-map-sequence-id-177629

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  8. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44078-plasmid-map-sequence-id-61691

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  9. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid
    #44090
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44090-plasmid-map-sequence-id-61689

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  11. pDEST47-ARF4-GFP

    Plasmid
    #67392
    Purpose
    Mammalian expression of hArf4 with C-term GFP tag
    Depositor
    Richard Kahn
    Publication
    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ARF4 (ARF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    V53A, L123P and M134I mutations were unintended P…
    Promoter
    CMV
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. MRGPRX4-Tango

    Plasmid
    #66442
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRGPRX4 (MRGPRX4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  13. MRGPRX3-Tango

    Plasmid
    #66441
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRGPRX3 (MRGPRX3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. YFP(159-238)-gamma-1 in pcDNAI/Amp

    Plasmid
    #54471
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(159-238)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54471-plasmid-map-sequence-id-115002

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  16. GPR101-Tango

    Plasmid
    #66301
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR101 (GPR101 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pBALU12

    Plasmid
    #69328
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Publication
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-mChOint-FgF
    Expression
    Bacterial
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1820 of 4673 results