PurposeMammalian expression of hArf4 with C-term GFP tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67392||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationV53A, L123P and M134I mutations were unintended PCR-generated artifacts
Entrez GeneARF4 (a.k.a. ARF2)
- Promoter CMV
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this plasmid contains the V53A, L123P and M134I mutations in ARF4 from the parent vector pDONR221-ARF4-no stop (Addgene plasmid # 67311). These mutations were unintentionally introduced during PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST47-ARF4-GFP was a gift from Richard Kahn (Addgene plasmid # 67392 ; http://n2t.net/addgene:67392 ; RRID:Addgene_67392)