Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,759 results for: nls

Showing: 1841 - 1860 of 18759 results

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pLKO_C.HA.Ollas_mCherry-NLS

Plasmid

#178239

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_C.HA.NWS_mCherry-NLS

Plasmid

#178238

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.NWS and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.S.V5_mCherry-NLS

Plasmid

#178235

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.S.V5 and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.Ollas.V5_mCherry-NLS

Plasmid

#178233

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.Ollas.V5 and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.Ollas.VSVg_mCherry-NLS

Plasmid

#178232

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.Ollas.VSVg and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.Ollas.S_mCherry-NLS

Plasmid

#178231

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.Ollas.S and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.NWS.V5_mCherry-NLS

Plasmid

#178230

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.NWS.V5 and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.NWS.VSVg_mCherry-NLS

Plasmid

#178229

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.NWS.VSVg and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.NWS.S_mCherry-NLS

Plasmid

#178228

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.NWS.S and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.NWS.Ollas_mCherry-NLS

Plasmid

#178227

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.NWS.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HSV.V5_mCherry-NLS

Plasmid

#178226

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.V5 and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HSV.NWS_mCherry-NLS

Plasmid

#178222

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.NWS and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HA.NWS_mCherry-NLS

Plasmid

#178217

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.NWS and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CDS12_Cas9-NLS

Plasmid

#136122

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_AtcoCas9-NLS(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCaggs-NLS-PAmCherry1-GSS-EGFP

Plasmid

#158000

Purpose

Expression of nucleus localized PAmCherry1 and EGFP fusion protein

Depositor

Francois St-Pierre

Publication

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

NLS-PAmCherry1-GSS-EGFP

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

Plasmid

#159945

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTFP1.0-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

Plasmid

#159975

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.F434L

Use

AAV

Expression

Mammalian

Mutation

F434L

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

Plasmid

#159973

Purpose

optical regulation of nuclear export with extended relaxation time

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.V416I

Use

AAV

Expression

Mammalian

Mutation

V416I

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

Plasmid

#159974

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.I427V

Use

AAV

Expression

Mammalian

Mutation

I427V

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

Plasmid

#139094

Purpose

Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Publication

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Showing: 1841 - 1860 of 18759 results

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