We narrowed to 12,750 results for: CAR

1. pJK175

   Plasmid

   #71132

   Purpose

   Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7

   Available since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-CANTHipi

   Plasmid

   #53301

   Purpose

   Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtO
   

   Use

   Low copy number bacterial cloning vector

   Tags

   6His

   Expression

   Mutation

   Promoter

   Trc

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Myc-ASC

   Plasmid

   #73952

   Purpose

   Express c-Myc-tagged human ASC in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 FLAG metap2

   Plasmid

   #32004

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   metap2 (Metap2 )
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-mCherry-CAAX PuroR

   Plasmid

   #166228

   Purpose

   Lentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   mCherry-CAAX
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307 ASC

   Plasmid

   #163977

   Purpose

   Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

   Depositor

   Daniel Bachovchin

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   ASC (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-NKX2.5

   Plasmid

   #46029

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   NKX2.5 (NKX2-5 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CaMKIIa-VChR1-EYFP-WPRE

   Plasmid

   #20954

   Purpose

   3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

   Insert

   volvox channelrhodopsin-1
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-GCaMP3bright

   Plasmid

   #74423

   Purpose

   Express fluorescent calcium indicator GCaMP3bright in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3bright
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D392A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdEasy-Msln-iCre-HA-Flag

    Plasmid

    #31305

    Depositor

    William Pu

    Article

    Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

    Insert

    Mesothelin (MSLN Human)
    

    Use

    Adenoviral

    Tags

    Flag and HA

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

13. mNeonGreen-Sec24D

    Plasmid

    #226581

    Purpose

    Encodes Sec24D protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

    Insert

    Sec24D (Sec24 homolog D) (SEC24D Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACM4-ACA

    Plasmid

    #49809

    Purpose

    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent site-directed mutagenesis to remove SalI s…

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. isoform PKC lambda (JCE590)

    Plasmid

    #89775

    Purpose

    yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Article

    Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

    Insert

    PKC lambda (Prkci Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. KTK_251

    Plasmid

    #180546

    Purpose

    Entry vector containing mScarlet

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS913.CB18.31

    Plasmid

    #69569

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-1 mice

    Depositor

    Francis R. Carbone

    Article

    Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

    Insert

    TCR beta (Tcrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. isoform PKC Alpha (JCE599)

    Plasmid

    #89779

    Purpose

    yeast expression of mouse PKC Alpha with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Article

    Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

    Insert

    PKC Alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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