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pGEX-6P-1-Eps8L2_1-496

Plasmid

#196516

Purpose

Expression of GST-Eps8L2_1-496

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Mutation

Silent mutations for siRNA resistance

Promoter

lac promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2_1-496-siR

Plasmid

#196515

Purpose

Expression of Myc-Eps8L2_1-496

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_1-183

Plasmid

#196535

Purpose

Expression of GST-Eps8L2_1-183

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_181-299

Plasmid

#196536

Purpose

Expression of GST-Eps8L2_181-299

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_544-715

Plasmid

#196540

Purpose

Expression of GST-Eps8L2_544-715

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2-FL-siR

Plasmid

#196514

Purpose

Expression of Myc-Eps8L2-FL

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2-FL-siR (EPS8L2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_529-715

Plasmid

#196533

Purpose

Entry vector for Gateway with Eps8L2_529-715

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_297-370

Plasmid

#196537

Purpose

Expression of GST-Eps8L2_297-370

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_367-496

Plasmid

#196538

Purpose

Expression of GST-Eps8L2_367-496

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_494-546

Plasmid

#196539

Purpose

Expression of GST-Eps8L2_494-546

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg101

Plasmid

#22876

Depositor

Noboru Mizushima

Publication

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Atg13-interacting protein (ATG101 Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Publication

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-C-CreintG

Plasmid

#69573

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - C-CreintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR v2-sgBAK-2

Plasmid

#210132

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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