We narrowed to 5,749 results for: actin
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Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-opa1 guide
Plasmid#121995PurposeAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expressionDepositorInsertopa1 gRNA
UseCRISPRAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA
Plasmid#168290PurposeCRISPR-activator Suntag-P65-HSF1 (SPH)DepositorInsertdCas9, Suntag-P65-HSF1
ExpressionMammalianPromoterCAGAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn
Plasmid#128485PurposeFluorescent reporter for serotonin (dendrite localization tag)DepositorHas ServiceAAV9InsertiSeroSnFR
UseAAVTagsFull-length neuroligin, Ig-kappa leader, and cpsf…ExpressionMammalianPromoterCAGAvailable SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_luciferase
Plasmid#103055PurposeEncodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbolavirus minigenome
UseLuciferaseExpressionMammalianPromoterCAGAvailable SinceDec. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorAvailable SinceDec. 20, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-EGFP
Plasmid#59331PurposeExpresses EGFPDepositorHas ServiceAAV1InsertEGFP
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP6-10gly-VPminx4
Plasmid#50796PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP6-VPminx4 activation domain fusion protein
ExpressionMammalianPromoterCAGAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ai38
Plasmid#34883DepositorInsertCAG-Floxed GCaMP3
UseMouse TargetingPromoterCAGAvailable SinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAK-2
Plasmid#210132Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
ExpressionMammalianPromoterCAGAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)
Plasmid#104571PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domainsDepositorInserthuman codon optimized ‘dead’ Cpf1 fused to four DmrA domains
UseCRISPRTagsNLS-3xHA-3xFLAG-4xDmrAExpressionMammalianMutationD832APromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-mTurquoise2
Plasmid#104059PurposeAAV expression of Cre-dependent reporter mTurquoise2DepositorInsertmTurquoise2
UseAAVPromoterCAGAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84261PurposeExpresses AND gate ABA/GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …ExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3
Plasmid#47874PurposeDestination plasmid with mClover for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmCloverExpressionMammalianPromoterCAGAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only