We narrowed to 14,886 results for: pET

1. pET_RGG-RGG-RGG

   Plasmid

   #124938

   Purpose

   Expression of RGG-RGG-RGG

   Depositor

   Benjamin Schuster, Daniel Hammer, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM-30-GST-PfAgo_F

   Plasmid

   #146265

   Purpose

   Bacterial Expression of PfAgo

   Depositor

   Elisa Izaurralde

   Insert

   PfAgo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one silent T1704C and one non silent mutation A90…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-RBP-LOV2

   Plasmid

   #185792

   Purpose

   Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-LOV2
   

   Use

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-HIS-Sangamo

   Plasmid

   #40786

   Purpose

   for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

   Depositor

   Gang Bao

   Insert

   Transcription Activator Like Effector Nuclease
   

   Use

   TALEN

   Tags

   N Terminal 6xHIS

   Expression

   Bacterial

   Mutation

   +63 C Terminus, Codon Optimized FokI Endonuclease

   Promoter

   T7

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. wt HIV-1 integrase in pET15b

   Plasmid

   #61668

   Purpose

   wild type HIV-1 integrase in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   wild type HIV-1 integrase
   

   Use

   Tags

   His

   Expression

   Mutation

   Promoter

   T7 promotor

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pETM-11-6His-DmBelle_B

   Plasmid

   #145950

   Purpose

   Bacterial Expression of DmBelle

   Depositor

   Elisa Izaurralde

   Insert

   DmBelle (bel Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET51b(+)_MBP-nLucCP-67/68

   Plasmid

   #113922

   Purpose

   Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   MBP-NanoLucCP-67/68
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-51b(+)-Caprola_13

   Plasmid

   #194674

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_13
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-Sac-Aβ(M1-42/C18C33)

   Plasmid

   #173759

   Purpose

   Expresses the AβC18C33 peptide.

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

   Insert

   Aβ(M1-42/C18C33) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed valine 18 to cysteine; changed glycine 33…

   Promoter

   T7

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b His6-ecDHFR (WT)

    Plasmid

    #73188

    Purpose

    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

    Depositor

    Thomas Wandless

    Insert

    folA dihydrofolate reductase (folA E. coli)
    

    Use

    Tags

    His 6X

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-Gate2 mazEF

    Plasmid

    #117117

    Purpose

    Destination vector with mazEF toxin antitoxin system

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Type

    Empty backbone

    Use

    Backbone for golden gate assembly for gene target…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet-1 Flag-LplA

    Plasmid

    #232118

    Purpose

    For bacterial expression of Flag-tagged E. coli LplA.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    lplA (lplA Escherichia coli (strain K12))
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET-51b(+)-Caprola_05

    Plasmid

    #194666

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Bst-LF-Idaho

    Plasmid

    #170469

    Purpose

    Expresses His-tagged Bst-LF-Idaho in E. coli

    Depositor

    Jonathan Schmid-Burgk

    Insert

    Bst-LF-Idaho
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge