We narrowed to 14,886 results for: pET

1. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-APOBEC1-YTHmut

   Plasmid

   #200159

   Purpose

   For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHmut
   

   Use

   Tags

   6xHis, HA, and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-pro-sumo1

   Plasmid

   #25101

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Serine 2 to Alanine

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pET30-T7-His-sfGFP

   Plasmid

   #180755

   Purpose

   pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Use

   Tags

   C-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDUET-GST AO7REwt

   Plasmid

   #213935

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET19b+H2B(T116C)

   Plasmid

   #157808

   Purpose

   Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H2B(T116C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T116C

   Promoter

   T7

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28-SpyCatcher002-MBP

    Plasmid

    #216295

    Purpose

    Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher002-MBP
    

    Use

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Mouse, Human, Bovine, Rat)
    

    Use

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 polymerase

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-AqpZ-ALFA

    Plasmid

    #212310

    Purpose

    Expression of AqpZ with ALFA tag

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    Aquaporin Z
    

    Use

    Tags

    ALFA tag and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-51b(+)-Caprola_01

    Plasmid

    #194662

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-HRASLS3-1-132

    Plasmid

    #100725

    Purpose

    Expression of N-terminal region of human HRASLS3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    HRASLS3 (PLAAT3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b(+)-SNAP-CLIP

    Plasmid

    #127180

    Purpose

    Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    SNAP-CLIP
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge