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Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99F
Plasmid#149439PurposeExpression of MIF Y99F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
UseTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21b-rrt-His8
Plasmid#194088PurposeExpresses Ec86 reverse transcriptase in Escherichia coliDepositorInsertEc86 rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3d-E2-25K-C170S
Plasmid#18892DepositorInsertUbiquitin conjugating enzyme E2-25K (UBE2K Most likely H. sapiens, Human)
UseTagsExpressionBacterialMutationCysteine 170 is replaced with a Serine residue.PromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_09
Plasmid#194670PurposeProtein production of the split HaloTag based calcium recorder Caprola_09 in bacteriaDepositorInsertCaprola_09
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(T17N)
Plasmid#22784DepositorInsertRac1 (T17N) (RAC1 Human, A. victoria)
UseTagsCyPetExpressionMammalianMutationRac1 T17N.PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc2
Plasmid#135629PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)DepositorInsertNAD-cpLuc2 sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag10
Plasmid#175520PurposeExpression of HaloTag10 in bacterial cellsDepositorInsertHaloTag10
UseTagsHisExpressionBacterialMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3521 pET ubcH6
Plasmid#8653DepositorInsertUbcH6 (UBE2E1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-IMX-DogTag
Plasmid#113765PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigaseDepositorInsertIMX-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GFP-CPDSalI
Plasmid#38257DepositorInserteGFP
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pEGFPN3 (Clonetech)PromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOV-Rsn2
Plasmid#61687Purposebacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOVDepositorInsertRanaspumin-2
UseTagsHis and iLOVExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only