We narrowed to 93 results for: dcas9

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

We highlight some exciting new deposits in our repository of plasmids and AAVs.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.