We narrowed to 33 results for: loxP

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

When using AAV for precise neuronal targeting consider AAV tropism and administration route, cell-type specific expression, and combining different approaches to increase targeting specificity further.

Stochastic multicolor labeling is a powerful tool for discriminating between neurons. Learn about how a new system named Tetbow (Tetracycline transactivator Brainbow) can bring brighter multicolor labeling to neuronal tracing experiments.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).