We narrowed to 219 results for: crispr c plasmids

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Learn how you can use the CUT&RUN method to study protein-DNA interactions.

Learn more about unique organisms that are being developed as model systems in this blog post.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Find bacterial expression plasmids that can be used for protein purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria.

Find tips on how to write your first review article - a useful experience for any scientist.

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

Learn how to produce, purify, and titer adenoviral vectors for your research.

Find tips for transforming arabidopsis with your plasmid construct of interest.