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We narrowed to 537 results for: try

Showing: 181 - 200 of 537 results

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...next section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational... to other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in ...

  4. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

  5. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...toolbox you are putting together?  Voigt: We’ve been trying to get everything to the scale of building synthetic...is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something has to be...program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon was built on...We did demonstrate that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...
Showing: 181 - 200 of 537 results