We narrowed to 963 results for: form

...problems that need to be resolved. If there are any forms that need to be signed, or manuscripts that need...

... of interest is not available in a pure form, you can perform relative quantification, which measures ...If your protein of interest is available in pure form, you can use it to create a standard curve on your...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...chemical-based. Before selecting one, check your antibody information. Many antibodies will have a recommended blocking...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...Aldridge et al., 2008).  Woah! That was a lot of information to cover. But don’t worry — the next time you...

...fill out our form to let us know what you would like to see available in viral prep format. Please note... protein, the virus can replicate but the newly formed viruses are unable to infect any neighboring cells... AAV can be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does...

...working day, including appropriate MTAs and customs forms. Of course, ordering the plasmids is the easy part...

...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice!   References and Resources References...

...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

...case, the larva will metamorphose into its adult form. In all, it takes roughly 10 days for an egg to ...Some resources will direct you to the latest information about gene function, while others offer fly stocks...Flybase.org is absolutely essential. You can look up information about your fly gene using the search box in the... started. Any given gene’s webpage will have information on where the gene is expressed within the fly...website as well. Additionally, they have useful information on topics such as choosing the right balancer...

...resides in and potentially which other proteins it forms complexes with. These “colocalization” studies give...

...transcriptional start site of a gene of interest, Cas9 forms a steric block that prevents RNA polymerase from...

...’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most fast-growing...

...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

...subtilis genome, the Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred...

...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

...the two halves are in close proximity, they will form the full FP and fluoresce. Split FPs can be used...

...antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation of recombinant...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies... Synthetic single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018...

...mechanism (see next section for details). The newly formed single-stranded DNA (ssDNA) is then pulled through...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously... D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell, ...

...expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added cofactors.      ...future MagLOV-based tools, just as light-induced conformational changes are the basis of many optogenetic tools...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

...secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... than mammalian cells, but mammalian cells can perform human-like post-translational modifications that... If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...ones, consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies.... This can be due to conformational changes in the antigen or lower detection sensitivity of the assay...S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion ...

...the recombinant protein may differ from the native form, which can affect antibody binding.  We hope that...

...ultracentrifuge. During the spin, the virions will form a band due to their high density, which can be collected...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...