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Showing: 201 - 220 of 960 results

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...our 'Split RNA' design, where the pre-processing form of crRNA is expressed and a tracrRNA (the helper... from a separate promoter. In the pre-processing form, the crRNA contains 30nt of the target spacer, but...will be processed (i.e., cleaved) into a shorter form that contains 20bp of the target spacer, or in another...Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA...and using Cas9-based genome engineering tools to perform different types of genome targeting. They are very...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation of recombinant...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies... Synthetic single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ... of interest is not available in a pure form, you can perform relative quantification, which measures ...If your protein of interest is available in pure form, you can use it to create a standard curve on your...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...chemical-based. Before selecting one, check your antibody information. Many antibodies will have a recommended blocking...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...Aldridge et al., 2008).  Woah! That was a lot of information to cover. But don’t worry — the next time you...

  5. AAVED

    Type
    Collection
    ...asking participants to share practical information in the form of data and protocols showing examples ...Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation Ben Deverman 2:00 PM Cre-DOG and Combinatorial...using the technology in the lab (e.g., controls to perform, recommended histology conditions, minimum/maximum...etc.), novel applications, or any other useful information. Experimental protocols or schematics. Examples...help contribute to this publication. Citation information for openly shared communication: Adeno-Associated...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice! References and Resources References Bondeson...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

  12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...case, the larva will metamorphose into its adult form. In all, it takes roughly 10 days for an egg to ...Some resources will direct you to the latest information about gene function, while others offer fly stocks...Flybase.org is absolutely essential. You can look up information about your fly gene using the search box in the... started. Any given gene’s webpage will have information on where the gene is expressed within the fly...website as well. Additionally, they have useful information on topics such as choosing the right balancer...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...
Showing: 201 - 220 of 960 results