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Showing: 201 - 220 of 965 results

  1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...findings from structural studies on the ribosome performed in his lab. RNA is a structural and functional...that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring structure.”...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation of recombinant...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies... Synthetic single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ... of interest is not available in a pure form, you can perform relative quantification, which measures ...If your protein of interest is available in pure form, you can use it to create a standard curve on your...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...chemical-based. Before selecting one, check your antibody information. Many antibodies will have a recommended blocking...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...Aldridge et al., 2008).  Woah! That was a lot of information to cover. But don’t worry — the next time you...

  6. Promega Plasmid Collection

    Type
    Collection
    ...hands, thanks to interchangeable ligands. HaloTags form a covalent bond with these synthetic chemical ligands...proteins interact, the subunits come together to form an active enzyme and generate a bright luminescent...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified...and shipped within 6–8 business days. For more information about the ordering process, check out our help...plasmids available at Addgene Find additional information about luciferase reporter assays (Link opens ...

  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice!   References and Resources References...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

  13. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...case, the larva will metamorphose into its adult form. In all, it takes roughly 10 days for an egg to ...Some resources will direct you to the latest information about gene function, while others offer fly stocks...Flybase.org is absolutely essential. You can look up information about your fly gene using the search box in the... started. Any given gene’s webpage will have information on where the gene is expressed within the fly...website as well. Additionally, they have useful information on topics such as choosing the right balancer...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...
Showing: 201 - 220 of 965 results