We narrowed to 262 results for: aav

an overview of qPCR and ddPCR copy number variant assays.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

Having trouble with your transductions? Find troubleshooting tips from Addgene scientist Leila Haery.

Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more.

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.

Learn about RNA interference and how you can use shRNA plasmids to knockdown gene expression!

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.