We narrowed to 268 results for: aav
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TypeBlog Post...low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al. developed a Cpf1...
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Light Sheet Fluorescence Microscopy
TypeBlog Post...Thy1-YFP-H mice). However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter... -
Mammalian RNAi Tools
TypeCollection... a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression... -
Adenovirus Guide
TypeGuide...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...viruses that belong to the Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations...how they got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4,...Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred choice among researchers...expression cassettes are often too big to be packaged in rAAV vectors. This is the case of some commonly used ...first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb) and is further increased in second... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA... -
CRISPR Activation: A Practical Guide
TypeBlog Post... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find... -
Adenovirus Plasmids
TypeCollection...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are... -
Distribution to Industry
TypeCollection...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ... -
Retrovirus Plasmids
TypeCollection...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus... -
Lentivirus ddPCR Titration
TypeProtocol...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs that ... -
CRISPR Plasmids - Prime Edit
TypeCollection... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9... -
CRISPR History and Development for Genome Engineering
TypeCollection... smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that...also makes it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies... -
Science Guides
TypeGuide...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More... -
Quick Guide to All Things Lentivirus
TypeBlog Post...Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral Vector Uses and Overview... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For...