CRISPR Plasmids - RNA Editing
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Collection
...capabilities but are small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with...
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Depositor Collections
TypeCollection...Protein Collection Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science... -
Hot Plasmids: Winter 2025
TypeBlog Post...Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al. developed a Cpf1... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...Thy1-YFP-H mice). However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter... -
Plasmid Collections
TypeCollection...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org... -
CRISPR Activation: A Practical Guide
TypeBlog Post... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find... -
Lentivirus ddPCR Titration
TypeProtocol...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus... -
Mammalian RNAi Tools
TypeCollection... a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...occasions, shRNAs are transiently expressed using either AAV backbones or species-specific expression plasmids... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs that ... -
Science Guides
TypeGuide...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More... -
Adenovirus Plasmids
TypeCollection...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9... -
Distribution to Industry
TypeCollection...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...