...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...viruses that belong to the Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations...how they got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4,...Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred choice among researchers...expression cassettes are often too big to be packaged in rAAV vectors. This is the case of some commonly used ...first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb) and is further increased in second...