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We narrowed to 304 results for: cas9

Showing: 221 - 240 of 304 results

  1. Modular Cloning Guide

    Type
    Guide
    ...CRISPR/Cas9 vectors expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9...mechanisms. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering...assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency...biology and multigene engineering, including CRISPR/Cas9 components. MoChlo: Modular Cloning Chloroplast ...CRISPR Arnold Driessen 96 plasmids (including CRISPR/Cas9 components) for expression or gene editing in filamentous...proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian...Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome editing in the yeast P. pastoris...

  2. Editor's Choice, October 2016

    Type
    Blog Post
    ...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... hampered by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially...: PMC3277219. 6. Ohmori, Tsukasa, et al. "CRISPR/Cas9-mediated genome editing via postnatal administration...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ...

  5. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...

  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...inserted the deaminase into the C-terminal domain of Cas9, which narrowed the editing window and reduced A...site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage the cell.... gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA to create...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop ... variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes...

  10. Editor's Choice, August 2016

    Type
    Blog Post
    ...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...

  13. Antibodies 101: Validation

    Type
    Blog Post
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  14. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed...PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...

  17. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)...are enriched in the 20 nm radius surrounding the dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology... technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted spatial...

  18. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... editing - all influenced by an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...
Showing: 221 - 240 of 304 results