We narrowed to 294 results for: cas9

PRIDICT 2.0 is an updated software that can help you design gRNAs for PRIME editing. Read more in our guest post!

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

The Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell lines.

The most viewed as well as editor's choice blog posts for October 2016: Changing Labor Laws and Building a Graduate Student Community.

Read our adeno-associated viral vector guide to learn about AAV vector components, production and common uses.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Learn more about unique organisms that are being developed as model systems in this blog post.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

Check out the top ten blog posts on the Addgene blog from 2019. Topics include CRISPR, fluorescent protein, plasmids, and more.

Blog editor Tyler Ford runs down some of the best posts from August 2016. NgAgo, site directed mutagenesis, and much more!

Antibody validation shows that your antibody is targeting exactly what it is supposed to. This post walks through 5 validation strategies.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.