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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 533 results for: try

Showing: 221 - 240 of 533 results

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...it was promising enough to keep exploring and to try developing a variant with an enhanced response. Evolution...researchers from a range of fields, like quantum chemistry and protein biophysics, as well as inventors and...effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...

  7. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...of saying “I have excellent communication skills” try giving an example, like “I have extensive communication... Should I write a cover letter? If you’re in a country where cover letters are common, the answer is usually...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover letters...if ever, sees cover letters submitted for their entry-level jobs and does not require or request them ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...positions. Many did not require cover letters for entry-level positions, as at that stage, cover letters...

  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...organisms, they can be problematic for researchers trying to express proteins in heterologous hosts. If you...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...applying for technical support positions, you should try to match your expertise with that of the company....are often found in sales or lab-based roles in industry. What to look forward to in a technical support...offer an easy transition from an academic lab to industry without being too commercial or “sales-y”. The...be a good jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    ...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...

  19. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...microfuge tube and vortex. Pro-Tip You may need to try a few different concentrations of antigen to determine...101: The Four ELISAs and When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification...
Showing: 221 - 240 of 533 results