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Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

Sarting a journal club? Read our guide to the logistics of forming a journal club and find different formats to hold the journal club discussion.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Addgene Outreach Scientist Klaus Wanisch provides us with all the details on a science careers in technical support.

Learn how microrganisms develop antibiotic resistance and how you can take some simple steps to combat them.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Protein tags can be used for purification, detection, localization, and more. Learn more about the molecular tool box of tags and fusion proteins.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Jim Woodgett discusses the data sharing policies of journals, such as PLOS, and how access to data affects scientific research.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol - How to Ligate Plasmid DNA

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

If flow plots seem confusing, our guide to understanding them is here to help!

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.