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...and to model diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells...Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors To date, the...group is an optimized mixture of three episomal plasmids (Table 1) encoding the reprogramming factors. ...enhance the reprogramming efficiency, an extra plasmid, pCXWB-EBNA-1 is used in the reprogramming cocktail...increases protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector...available at Addgene. Table 1: List of pCXLE episomal plasmids, their Addgene ID numbers and the reprogramming...reprogramming factors they encode Episomal Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL...

...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

...Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place of the epitope...Jürg Bähler have deposited a large collection of plasmids for genome modification in yeast developed by ...Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome modifications in ...Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional ...

...from our repository to find our most requested plasmid in 2015. In line with all of the exciting developments... past year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

...Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources...Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1....combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-... plasmid cloning...

...relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids in the...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral... virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on donations. That seed...depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently sharing viral preps) through...positive response to the option of Addgene providing plasmids in a ready-to-use viral format. Having identified.... With their expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

...mutation” field on the plasmid page (see plasmid 87904 for example). As many plasmids available from Addgene...blog Plasmids 101: protein expression Plasmids 101: E. coli strains for protein expression Plasmids 101...codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene...

... How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, ... Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine) pTP955...Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice ...column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: Purified nanobodies...Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M... on Addgene.org Find more nanobody expression plasmids here Additional Resources Looking for monoclonal...

... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid 101: Repressible Promoters CRISPR...knock out. Looking for some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector...of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn it on...

...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported active DNA...increase in transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve...

...) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight some...popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging, check...the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles...

...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and... contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

...and to make the desired genome edit:  A donor plasmid that contains a puromycin resistance gene linked...the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The Madison lab also created...in the reporter gene). A control RFP expression plasmid.  With this set up, all successfully transfected... and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP expression, presumably...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software ...