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We narrowed to 893 results for: plasmid

Showing: 261 - 280 of 893 results

  1. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ... ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV genome, ITRs serve as...gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ...molecule as the gene that they package. In a transfer plasmid, the transgene is flanked by two ITRs, and the ...genome of AAV in the native virus. In an AAV vector plasmid, they flank the gene of interest. A single ITR ...growth culture and result in a high percentage of plasmid DNA with mutated ITR sequences. Using recombinant-deficient...located immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these...

  2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

  3. Plasmids 101: Codon usage bias

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    Blog Post
    ...mutation” field on the plasmid page (see plasmid 87904 for example). As many plasmids available from Addgene...blog Plasmids 101: protein expression Plasmids 101: E. coli strains for protein expression Plasmids 101...codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene...

  4. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ... How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, ... Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine) pTP955...Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice ...column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: Purified nanobodies...Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M... on Addgene.org Find more nanobody expression plasmids here Additional Resources Looking for monoclonal...

  5. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    ...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported active DNA...increase in transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve...

  6. Plasmids 101: The protein expression toolbox

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    Blog Post
    ... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid 101: Repressible Promoters CRISPR...knock out. Looking for some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector...of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn it on...

  8. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight some...popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging, check...the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution...

  10. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

  11. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

  12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and... contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

  13. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  14. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...and to make the desired genome edit:  A donor plasmid that contains a puromycin resistance gene linked...the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The Madison lab also created...in the reporter gene). A control RFP expression plasmid.  With this set up, all successfully transfected... and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP expression, presumably...just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent...embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software ...

  16. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ... of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria links the...encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...editors for your research Read our CRISPR guide Find plasmids for your research ...

  17. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check...

  18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...284 . https://doi.org/10.1038/nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD,... https://doi.org/10.1126/science.aaq0180 Find plasmids from this paper at Addgene. Kim D, Kim J, Hur...863–868 . https://doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo ...8: . https://doi.org/10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver...485 . https://doi.org/10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver...869–874 . https://doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller...191 . https://doi.org/10.1038/nature14299 Find plasmids from this publication at Addgene. Walton RT, ...

  19. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts...origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll...the promoter, which is necessary for getting the plasmid express our gene of interest. This element is responsible.... This means that the promoter present in your plasmid backbone must to be compatible with the type of...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism...development-specific promoters available to scientists. Plasmids are oftentimes put to therapeutic uses, and in...Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about...

  20. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...Addgene.org Find Fluorescent Protein Plasmids Check Out Other Hot Plasmid Technologies ...proteins and is driven by an inducible promoter (Plasmid 64542 or 64543) Before translation, both GFP and...19213877 PMCID: PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation...1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, ...976-89. PMID: 27153498 PMCID: PMC4889334 Find plasmids from this paper at Addgene. Additional Resources...
Showing: 261 - 280 of 893 results