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We narrowed to 533 results for: try

Showing: 261 - 280 of 533 results

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...expression in a variety of cell types, but you should try to find the best AAV for your particular experiment...: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15 (2005): 9933...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...spread from the injection site it is advisable to try a few different injection titers and volumes to determine...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https:/...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...tissue tropism for mice in vivo. To target... Try using... Citation CNS AAV1, AAV2, AAV5, AAV8, AAV9... for gene transfer. The Journal of Biological Chemistry, 276(23), 20610–20616. https://doi.org/10.1074...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...enough to go up a kit size, it may be tempting to try increasing your input to increase your output. But...quantity and quality of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires...than half 70% recycled materials Sustainable forestry certification Starch-based compostable bags for...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...its substrate. Then, all it takes is a bit of chemistry magic to produce a wide range of useful molecules...incorporate an alkene to be labeled through click chemistry. Although these multi-component systems can be...brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and HaloTag ligands...Tag for Multiprotein Labeling in Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016...L. (2016, September 8). Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https...https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Gene does not appear in the auto-populated list, try entering the gene's Official Full Name or one of ...the top right of each data entry page. Once you have completed the data entry process for all of the plasmids...will be saved and the page will open a new set of entry fields to describe the next insert. Repeat as needed...the "Submit Deposit" button to complete the data entry. Clicking the "Submit Deposit" button will allow...

  11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). ...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...analyzed for tagging efficiency using standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients...single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069...

  12. COVID-19 Resources

    Type
    Collection
    ...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...this novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on ...plasmids that have been used for measuring viral entry and activity in neutralization assays and links ...growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV...-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV ... as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find plasmids related...

  13. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry Blues Plasmid...Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry Greens Plasmid...Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description Industry Do you...

  15. Plan Your Experiment

    Type
    Guide
    ...identify the genomic sequence for the gene you are trying to target. However, the exact region of the gene...

  16. Deep Dive: qPCR

    Type
    Blog Post
    ...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)   Chemistry Background noise Probe Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...

  17. Cre-lox system

    Type
    Collection
    ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547...Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral...components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2...139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant...

  18. Genetic Code Expansion

    Type
    Collection
    ...Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp), 5-Fluoro-L-Tryptophan (5FTrp) Bacterial TAG Thomas Huber...Methanogenic archaeon 6-Fluoro-L-Tryptophan (6FTrp), 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG Thomas Huber...pRSF-G1(7FTrp)RS PylRS M. archaeon 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG Thomas Huber 177311 pRSF-G1TFAKRS...7AW)RS G1(7AW)RS Methanogenic archaeon 7-Aza-L-Tryptophan (7AW) Bacterial TAG Thomas Huber 207620 pRSF-...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...activation domain is an incredible tool for researchers trying to activate target genes, the relatively large ...to customize for targeting your gene of interest. Try them today! Ordon J, et al. Plant J. 2016. PubMed...food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend on the yeast... pull-down methods and identified using mass spectrometry. The Roux Lab’s new and improved version of ...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Jul 29;7:12266. David Savage Tryptophan Visualize intracellular tryptophan levels and flux Nanosensor detection...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS One. 2015 Apr 14... Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. Martin Webb Phosphate... detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10)... Grx1-roCherry. Redox Biol. 2019 Feb;21:101071. Dmitry Bilan Temperature Genetically encoded ratiometric...
Showing: 261 - 280 of 533 results