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We narrowed to 304 results for: cas9

Showing: 281 - 300 of 304 results

  4. Lentiviral Vector Guide

    Type
    Guide
    ..., J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science , 343...

  5. Fluorescence Titering Assay

    Type
    Protocol
    ...transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed...

  6. Lentivirus Production

    Type
    Protocol
    ...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ...serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with...

  8. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome...

  9. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also driven...piggyBac gRNA delivery and the all-in-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene...approaches rely on continual expression of both dCas9 and the gRNA. This expression can vary significantly...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR... could quantify and track cells expressing both dCas9 and gRNAs. The multi-gRNA piggyBac vector ...plasmids for piggyBac gRNA delivery and the all-in-one dCas9 targeting plasmids! Hazelbaker et al., Sci Reports...

  11. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses FnCas9, a protein...sensitivity of FnCas9, this is also a good way to diagnose single nucleotide variations. Also, FnCas9 cleaves...field-deployable nucleobase detection and identification using FnCas9 (FELUDA) Sensitivity: – 110 femtomolar  Time: Not...streptavidin coated beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches...field-deployable nucleobase detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...miniCRISPR” version, are much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of...

  16. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader...promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi is stably...

  18. The 12 Days of CRISPR

    Type
    Blog Post
    ...🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in...
Showing: 281 - 300 of 304 results