We narrowed to 602 results for: sam

1. The AAVantages of AAV in CRISPR Screens

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   ...vivo. The general process of these screens is the same: Deliver CRISPR library to cells of interest. Select...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...

2. Production of Virus in Insect Versus Mammalian Cells

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   ..., so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...P1 virus, so that if you ever need to remake the same virus, you can skip the first round of production...

3. Viral Vectors 101: Producing Your rAAV

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   ...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...

4. Pooled Library Amplifications

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   ...library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual... all of the bacteria can grow and compete in the same container, making it easier for a smaller, recombined...

5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

6. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ... 2-3). Since donor plasmids of the same type will produce the same unique SapI 5’ overhang, different ...

7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...achieved by isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-...in a second paper published in Nature Methods the same year that delivering RNA-guided nucleases or TALENs...

8. 10 Basic tips for mammalian cell culture

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   ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

9. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...phenotype since additional genes that can do the same function are less likely to exist). If there’s an...to get males to recombine two mutations onto the same chromosome. You should use females for that. Now...

10. Addgene's Cancer Collection Pages Connect the Research Community

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    ...brake pedal that does not work. Both cases yield the same result – uncontrolled movement of the vehicle or...transcript most commonly found across all TCGA tumor samples is represented, making the results of any such ...

11. Anatomy of a Plasmid Page at Addgene

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    ...from the same lab while the publication page contains all the plasmids deposited from the same publication...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...Our hope was that anyone else who came up with the same idea or wanted to build a gene drive in a model ... an appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental...

13. Plasmids 101: Secondary Nanobody Toolbox

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    ...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...

14. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...paying bills and having a life outside my job. The same can be said about teamwork, communication, writing...process. You stepped up and got your team on the same page. This came naturally to you. How is this new...

15. Uncovering Ancestral Bias in CRISPR Research

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    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange... on these and any other CRISPR screens using the same reference genomes and libraries. Further complicating...

16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...seven required genes and the CRISPR RNA under the same promoter, and a donor plasmid (pDonor) encodes the...immunity, whereby multiple proximal insertions with the same transposon exhibit reduced efficiencies (Vo et al...

17. AAVs in Retinal Gene Therapy

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    ...retinal cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...

18. MXS Chaining

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    ...e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate each... individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled...

19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii are genetically...very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve...

20. Tips for Screening with Yeast Two Hybrid Systems

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    ...libraries of bait and prey against each other at the same time in order to try and access the entire interactome...the protein partner or the UAS/reporter gene, the same bait and prey libraries can be screened using both...