We narrowed to 912 results for: plasmid

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

In the final post of our Quick Guide to Working with Drosophila, learn how to make Drosophila transgenic lines.

Addgene spoke to Kiran Musunuru about his new genome editing TALEN kit, the research the kit now makes possible, and how it compares to his CRISPR/Cas9 system.

Learn how Addgene is supporting rare disease research through the Rare Genomics Institute and the BeHeard Award 2017.

Addgene recently attended the ASM Microbe 2016 conference in Boston. Check out what we learned!

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Addgene scientists perform standard quality control for AAVs. But we are also going one step further with next-generation sequencing (NGS) of the viral genome.

Science educator, rapper, and communicator Tom McFadden gives us SciComm tips and tell us all about science outreach company, Science with Tom.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.