We narrowed to 441 results for: %s

1. Cre-Lox and Other Site-Specific Recombinases

   Type

   Collection

   ...recombinases derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. Other tyrosine recombinases...

2. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   ...following four questions to figure out which method(s) work best for you, your audience, and your science...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   ...PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   ...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)...

5. Pooled Library Amplifications

   Type

   Blog Post

   ...and how many cells to use for your transformation(s). The amount of competent cells you need total and...

6. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding...

7. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications...

8. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

10. DNA Purification Without a Kit

    Type

    Blog Post

    ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...

11. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

14. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...

15. Bioinformatics at Addgene

    Type

    Blog Post

    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

16. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

19. MXS Chaining

    Type

    Blog Post

    ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

20. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...