We narrowed to 438 results for: %s

1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   ...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)...

2. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   ...following four questions to figure out which method(s) work best for you, your audience, and your science...

3. Pooled Library Amplifications

   Type

   Blog Post

   ...and how many cells to use for your transformation(s). The amount of competent cells you need total and...

4. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding...

5. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications...

6. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

7. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

8. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

9. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

10. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

11. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

13. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

15. MXS Chaining

    Type

    Blog Post

    ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

16. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

18. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

20. Bioinformatics at Addgene

    Type

    Blog Post

    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...