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1. Your Lentiviral Plasmid FAQs Answered

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   ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

3. New Tools Enable CRISPRa for Neuroscience Applications

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   ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

4. Isolating Genomic Regions of Interest with the CRISPR System

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   ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

5. Plasmids 101: Codon usage bias

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   ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

6. AAVs in Retinal Gene Therapy

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   ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

7. Analyzing CRISPR Editing Results with ICE from Synthego

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   ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

8. Bioinformatics at Addgene

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   ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

9. New CRISPR Tools: Cas7-11 and PASTE

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   ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

10. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

11. Fluorescent Proteins 101: Fluorescent Protein Timers

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    .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

12. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

13. Plasmids 101: Shuttle Vectors

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    ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

14. Hot Plasmids - November 2023

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    ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...

15. How to Be an Excellent Trainee

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    ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

16. Viral Vectors 101: Preparing Pooled Libraries

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    ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

17. Getting to Know Expansion Microscopy

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    ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

18. Great Results Start with Great Standard Curves

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    ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

19. Plasmids 101: Secondary Nanobody Toolbox

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    ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

20. Transferable Skills Guide: Career Planning Resources

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    ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...