We narrowed to 957 results for: Cre

...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...Conditional overexpression plasmid. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and...largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells. See the Weinberg Lab page...pBABE-hygro-hTERT MoMLV Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin...cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE... post-transcriptional regulatory element that increases export to the cytoplasm. William Hahn 11375 MDH1...

... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...

...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...antibodies to zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene...American Zebrafish Network (LAZEN) - LAZEN aims to increase the use of zebrafish as a model organism and to...

...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available....: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids Browse our selection...

...for creating a glycerol stock, including additional tips for long-term storage.... Protocols Creating Bacterial...Glycerol Stocks for Long-term Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage...store your plasmid DNA at -20°C, many labs also create bacterial glycerol stocks of their plasmids. This... Watch the protocol video below to learn how to create bacterial glycerol stocks. Procedure Follow the...overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes: Make...

...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...including plasmids for constitutive, conditional (Cre-lox), or inducible (Tet) expression miRNA and 3' ...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...some experiments, scientists often want to create stable cell lines in which the expression cassette... your regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter...

...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors...

...Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent ...libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections A modular...

...inhibition. For example, search for "ChR mCherry", "Cre inhibition", or other terms. Looking for viral preps...channelrhodopsin have been engineered or identified to increase the spectral and kinetic properties as well as...

... pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression...pLV-hTERT-IRES-hygro 3rd Lentiviral expression of hTERT, used to create immortalized cell lines. See Hayer et al. (2016...transcription factors for identification in pooled screens. Part of the MORF Collection . Feng Zhang Return...Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases viral titer. Sergey Kasparov 22500 pNHP 2nd Contains...

...RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are...

... Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence (mRFP1) Escherichia...our CRISPR Plasmids and Resources page. Check our Cre-Lox Plasmids Collection for a selection of plasmids...Guide CRISPR Guide Bacteria are commonly used to create, store, and replicate plasmids of all types, but...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...benzoate (cumate) Sphingomonas sp. , Caulobacter crescentus , Paracoccus denitrificans , Methylobacterium...

...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid..._CEBPA_1 PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...genome modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in...has developed a systematic and scalable method to create marker-free mutations, insertions, and deletions...stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor plasmids containing ...

...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ...considerations, and more. CasPEDIA is a community resource created and maintained by the Jennifer Doudna Lab . Read...

...PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...kDa and 36 kDa, respectively). And they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments... kDa), very bright, and ATP-independent, and a secreted version of the protein is available, making it... regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise noted, ...Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly, Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase...

... and the screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured...CRISPR screen data that might be able to help you out. What is the Open Repository of CRISPR Screens? The...BioGRID) in order to increase the accessibility of CRISPR-based phenotypic screen datasets. Original published...BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot updated 2024.   Using the Open...Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell lines, each... browse through screens performed in relevant cell lines or conditions. This increased ease of access ...cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. ...

...tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox system is a technology ...marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing Cre downstream...constructed. Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully...generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established research ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...development, or by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be ...facilitate the use of Cre-lox technology, transgenic mice have been constructed that express Cre under a variety...

...be to inject your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre expression is controlled...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression... could breed your Cre-dependent mCherry line with a separate line that expresses Cre in neurons, but both...expression of any Cre-dependent gene of interest in neurons. This ability to be paired with any Cre-controlled...interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course there are also ...AAV that became available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150...150 orders since coming online! pmSyn1-EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde ...

...LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific...control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how...the control of Cre recombinase are used in combination with mouse lines that express Cre only in the cell...Tamoxifen inducible Cre versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible...about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox...contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase...

...Although Cre isn’t a modular protein, Tang et. al were able to create a split version of Cre that is only...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP ... of interest blocks transcription when Cre is absent. When Cre is present, it removes the STOP cassette...when dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives... cord. Cre-DOG is also suitable for optogenetic studies, and since cells infected with AAV-Cre-DOG retain... it is also possible to create split versions of non-modular proteins like Cre. Could Cas9-DOG be developed...