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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 98 results for: EGFP

Showing: 21 - 40 of 98 results

  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New serotype AiP13044...Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... by the temperature. For instance, enhanced GFP (EGFP) was optimized for 37°C, and is therefore most suited...many cases the brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g.... Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers (FT): These proteins...Fluorescence Spectrum Viewer  from BD bioscience. ilovegfp – a site with very comprehensive data sheets on...

  7. New Viral Vectors - March 2024

    Type
    Blog Post
    ...vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility to be used as internal tags due... normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias et al., 2002). GFP needs oxygen...original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness (Tsien, 1998...variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made by introducing two mutations...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...et al 2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...be conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...caused by T cell migration.  CAR expression (or the eGFP proxy for CAR) is elevated at stable levels for ...      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications ...
Showing: 21 - 40 of 98 results