We narrowed to 294 results for: cas9

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Discover the tools you need to perform plant genome engineering with CRISPR.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

CRISPR Cas9 gRNA plasmids from the Zhang lab

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.