We narrowed to 63 results for: gateway

1. Resolute Plasmid Collection

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   Collection

   ...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   ...Additional resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies ...

3. Plasmids 101: What is a plasmid?

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   Blog Post

   ...methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...

4. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   ...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows ...

5. Microbiology Resources

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   Collection

   ... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

6. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   Blog Post

   ...chemical stimulation was sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on...

7. CRISPR 101: Multiplex Expression of gRNAs

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   Blog Post

   ...repression), and Fok1-dCas9 (dimeric nuclease). Gateway assembly method Frew Lab Multiple Lentiviral Expression...should be compatible with type IIS enzyme BfuAI. Gateway cloning is then used to combine the multiple gRNAs...

8. Pushing the Limits of DNA Assembly

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   Blog Post

   ...Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology...

9. Plasmids 101: Blue-white Screening

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   Blog Post

   ...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ...

10. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility allows it...

11. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many...

12. 27 Hot Plasmids from 2016

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    Blog Post

    ...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...

13. Click Biology: How to Click and Play to Explore Biology

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    Blog Post

    ...expression C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega...

14. Plasmids 101: Protein Expression

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    Blog Post

    ...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...et al. Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog...

16. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, ...

17. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...

18. Zebrafish Plasmid Collection

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    Collection

    ...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...

19. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu...

20. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest...