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We narrowed to 966 results for: lat

Showing: 21 - 40 of 966 results

  1. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...humans. Then, one of them heard about our online platform, called Crowdfight, and filed a request for help...Crowdfight-COVID19 Crowdfight was born as a collaborative platform to help researchers working on COVID-19. Its origins...feeling helpless about the situation. But a few days later we had an idea. And this idea rapidly grew and became... became Crowdfight COVID-19, an online platform to connect researchers working on COVID-19 with scientists... designers and other professionals, joined the platform; while they are a minority, their help has been... to push certain projects. The idea behind the platform was simple: any person working on COVID-19 could...simpler and the volunteers helped with things like translations, bibliographic searches, etc.     Expanding...

  2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...tagless protein purification as well as reversibly regulation of endogenous signaling activity in cells.  Let...spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle...Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling...results demonstrate that OptoNBs can be harnessed to regulate signaling pathways such as the Ras/Erk pathway...

  4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...!   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool... slightly smaller population, something else was also suddenly cool: DNA methylation. In 1983, Andrew ...these studies report correlations without clearly testing whether a certain methylation state causes a condition...cause demethylation?  Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy methylation marks...site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked by ...the original methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization...allowing one to study the causal relationship between DNA demethylation and gene expression (Sapozhnikov...

  5. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ... Congenital Disorders of Glycosylation (APCDG) and an international platform for CDG research, named the...NFIB- and NFIC-related human syndromes. As therapies are developed for specific NFI-related genetic disorders...on rare diseases through the BeHEARD Award. Congratulations to Paula Videira, Michael McMurray, and Richard...Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...development. Paula Videira: Congenital disorders of glycosylation Paula Videira is an Assistant Professor at the...CDG&Allies-PPAIN).  Congenital disorders of glycosylation (CDG) are a group of rare inherited metabolic...Immunological aspects of congenital disorders of glycosylation (CDG): a review. J. Inherit. Metab. Dis. 39,...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells LAT1, pp36 LCK lymphocyte-specific ...ADRM1 adhesion regulating molecule 1 ARM1, GP110, MGC29536, Rpn13 AP3B1 adaptor-related protein complex...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence... IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated...kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory subunit...JULF2, KPG_004, NOP9 PF4 platelet factor 4 CXCL4, MGC138298, SCYB4 PF4V1 platelet factor 4 variant 1 CXCL4L1...

  8. Plan Your Experiment

    Type
    Collection
    ...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers... Select Your Desired Genetic Manipulation Different genetic manipulations require different CRISPR components... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the edit...modifications . Cell Population The first step of validation is determining the type of cell population you need ...you will likely need a clonal population. Additionally, clonal populations are useful for determining if...edited alleles. A clonal population is not always required, and a mixed population can be sufficient for ...

  9. CRISPR Guide

    Type
    Collection
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...modifying the methylation state of cytosines in a gene’s promoter or by inducing histone acetylation or demethylation...through increased H3K27 acetylation TET1 - activation through cytosine demethylation DNMT3A - repression ...repression through cytosine methylation MQ1 - repression through cytosine methylation with improved kinetics LSD1...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...generate the new phosphorylated protein. Protein Phosphorylation Protein phosphorylation is one of the most...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code... serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“... most abundant forms of posttranslational modification in cells and research into its many roles in protein... , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1* (V70) (Plasmid #188537) .... with the optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were ...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP...Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated vesicles Clathrin light chain GFP Bo Huang...Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Michael Davidson 79997 pmiRFP703...TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko 22479 FUmGW Membrane Palmitoylation GFP Connie Cepko...Membrane Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membrane Palmitoylation sequence... EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes...

  12. Antibody Guide

    Type
    Collection
    ...produces a colored product that accumulates over time which is relative to the amount of enzyme present...quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific ... one type of antibody. Monocultures of B cells isolated from animals immunized as described above are ... regions of the antibody to be independently manipulated. Antibodies produced this way are called recombinant... used while suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...instead of modified antibodies and are not closely related to antibodies. Monobodies - Monobodies are synthetic...

  13. mTOR Pathway

    Type
    Collection
    ...enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers....RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of...arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...determine the nutritional state of the cell and regulates the switch from anabolic to catabolic processes...Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...Akt and MAPK/ERK, both of which are commonly dysregulated in cancer. Loss of the tumor suppressor p53 ... caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer...

  14. Ras Pathway

    Type
    Collection
    ...(ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF...have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate group...GTPases play prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and...differentiation and survival. Ras is essential for regulating the growth of the cell, however when mutations...for clarity and does not indicate a specific relationship. The content and map for this page were generated...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...

  15. Brain Initiative Collection

    Type
    Collection
    ...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2...

  16. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...include histone acetylation by p300, histone demethylation by LSD1, cytosine methylation by DNMT3A or MQ1...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...100 bp window. This technique creates diverse populations of mutants for directed evolution. ID Plasmid...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker...

  17. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...solution later in this protocol. Isolating a Monoclonal Cell Population by Limiting Dilution Isolate cells...Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell Population by Limiting Dilution You may also...solution will be used to seed the 96-well plate. Sample Calculation First, use a hemocytometer to quantitate...cells/mL To seed one 96-well plate, make 10 mL of a 5 cell/mL solution. Calculate the total cells needed: ...into each well of a 96-well plate. By doing this, you are seeding the plate at an average density of 0.5... will help you focus the microscope later when scanning the plate for individual cells, which can be difficult...scan the plate for individual cells, which should be on the same focal plane. Scan the entire plate and make...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of previously...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-throughput...emerged as a model system for human disease and translational research, including the study of cancer, inflammation... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish...organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...

  19. Rett Syndrome

    Type
    Collection
    ... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...modifiers suggests the major function of MECP2 is to regulate gene expression either locally or globally. The...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

  20. p53 Pathway

    Type
    Collection
    ...through both phosphorylation and acetylation, which disrupt its interaction with negative regulators, increase...Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9...allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and does... does not indicate a specific relationship. The content for this page was generated with the help of .... CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...
Showing: 21 - 40 of 966 results