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We narrowed to 898 results for: plasmid

Showing: 401 - 420 of 898 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources References..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  5. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...topic or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

  6. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe... autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana... Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent protein guide: Biosensors...

  8. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

  9. What the HEK?

    Type
    Blog Post
    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...

  11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ....cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova...

  13. CRISPR Cheat Sheet

    Type
    Blog Post
    ...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

  14. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...

  15. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...

  16. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...enough. For example, if we need to develop a new plasmid collection page, we certainly don't expect that...design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here!        ...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...Reference Read about genetic code expansion Browse our plasmid collection page  ...

  18. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...

  20. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...
Showing: 401 - 420 of 898 results