We narrowed to 534 results for: try

1. Antibodies 101: Normalization and Loading Controls for Western Blots

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   Blog Post

   ...presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J. E., & Gomes, A. V., “Stain-Free...control to β-actin for Western blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j....

2. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   ...applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC... a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-...

4. Five Popular Model Organisms, Part 2

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   Blog Post

   ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

5. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...

6. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   ..., label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques...-based Photoactivatable Transcription System. Chemistry & Biology 22:169–174 . https://doi.org/10.1016...

7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

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   Protocol

   ...detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens in cells...

8. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

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   Protocol

   ... Transfection for Recombinant Antibodies Immunocytochemistry Protocol Western Blot Protocol Introduction...

9. Introducing the Addgene Antibodies 101 eBook!

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   Blog Post

   .... If you’re looking to design your first flow cytometry experiment, “Chapter 7: Sorting” is there to help...

10. Lucky Thirteen: Guest Contributors of 2023

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    Blog Post

    ...McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips for Getting a Faculty ...

11. Antibody Data Repositories and Search Engines

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    Blog Post

    ...Differentiation Antigen Workshops Immune cells Flow cytometry (potentially others) Data repository From ...

12. Podcast: A Malawian Professor's Path to Biotech Research

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    Blog Post

    ...one of the best of the best students in his home country. He was selected as one of 600 scholars among 100,000...

13. Addgene Stands with the Scientific Community

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    Blog Post

    ... seeking and expanding our collaborations with industry and public and private partners to create additional...

14. How Exercise Purges Stress and Keeps Depression at Bay

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    Blog Post

    ...clue: differences in the kynurenine pathway of tryptophan degradation. It turns out that activation of ...

15. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post

    ...was recognized in 2008 when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for...

16. Addgene's Top Blog Posts from 2020

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    Blog Post

    ...reagents available to scientists in both academia and industry. This year, we also launched the cloning grade...

17. Editor's Choice, September 2016

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    Blog Post

    ...Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer...

18. Editor's Choice, August 2016

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    Blog Post

    ...for another part of my job, I travel around the country and set up meetings with PIs to discuss the benefits...

19. Future of Research Conference - Remarkable Opening Session

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    Blog Post

    ...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

20. Addgene is Expanding Our Viral Vector Service!

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    Blog Post

    ...USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan to receive...