Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 541 results for: try

Showing: 401 - 420 of 541 results

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...

  10. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... blots, ELISAs, immunoprecipitation, and flow cytometry. The better these tools are, the better the reproducibility...cells. This enables direct screening via flow cytometry for improved binding under defined environmental...

  13. Molecular Cloning Techniques

    Type
    Guide
    ...via a proprietary BP clonase, creating an entry clone. The entry clone now has recombined attL sites flanking...made an entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors...collection contains thousands of Gateway donor , Gateway entry , and Gateway destination vectors with different...reducing ligation efficiency. TOPO-ready Gateway entry plasmids are also available, allowing for rapid ...

  15. Molecular Biology Reference

    Type
    Guide
    ...acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and purification...select or sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. Empty Backbones...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...

  17. Promoters

    Type
    Guide
    ...metabolic operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Constitutive Hybrid... The trp operon involved in the production of tryptophan is an example of a positively-controlled operon...
Showing: 401 - 420 of 541 results