We narrowed to 444 results for: crispr
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TypeCollection...also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important ...
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Lentiviral Vector Guide
TypeGuide...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science... -
Five Popular Model Organisms
TypeBlog Post... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous... -
Plasmids 101: Biotinylation
TypeBlog Post... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed... -
Evolution of Lab Techniques
TypeBlog Post... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation... -
Molecular Biology Reference
TypeGuide...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for... -
Adeno-associated virus (AAV) Guide
TypeGuide... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for... -
Hot Plasmids: Summer 2024
TypeBlog Post...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9... -
Quick Guide to All Things Lentivirus
TypeBlog Post...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs... -
Antibody Validation for Flow Cytometry
TypeBlog Post...expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO... -
Adenovirus Guide
TypeGuide...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...