Skip to main content
Addgene
Showing: 421 - 440 of 892 results

  3. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References...

  4. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

  5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...viruses, but you can find a list of validated gRNA plasmids on our website. There are a few reasons to target...18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described... PubMed Central PMCID: PMC4486245.2. pXPR_011 (Plasmid #59702) was a gift from John Doench & David Root...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...

  13. To Each HIS Own

    Type
    Blog Post
    ... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...on Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan...

  15. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...

  19. Editor's Choice, August 2016

    Type
    Blog Post
    ... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

  20. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This...
Showing: 421 - 440 of 892 results