We narrowed to 913 results for: plasmid

Learn how to determine the practical brightness of your fluorescent protein in your specific experimental setup.

Nano-lanterns contain luciferase fused to a fluorescent protein. The Nagai lab has assembled multicolored nano-lanterns for various applications.

Antibody validation shows that your antibody is targeting exactly what it is supposed to. This post walks through 5 validation strategies.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

Addgene is a non-profit organization that facilitates life science research. Learn more about how we've maintained our close-knit culture as we've grown.

Addgene is hosting #AddgeneCRISPRweek on Twitter. Check it out for anything from CRISPR basics to new CRISPR tools.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

Addgene celebrates 15 years of scientific sharing. In this post, executive director Joanne Kamens reflects on the past and future of Addgene.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Get a behind-the-scenes glimpse of the design behind Addgene's new homepage and mascot update.

Learn how you can use acoustic reporter genes for in vivo imaging experiments. These are good alternatives to fluorescence reporters.

Learn how you can use fluorescent biosensors to detect small molecules and other compounds of biological significance in your experiments

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Addgene is celebrating our 20th anniversary!

Learn how the Journal of Emerging Investigators enables high school students to learn more about the scientific process and scientific publishing.

A review of alternative PAM and PAM-flexible Cas enzymes.