We narrowed to 899 results for: plasmid
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TypeBlog Post....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...
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CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...procedures. The video details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected... PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...thanks for depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...was to make my own glass milk for purifying cut plasmid DNA from agar slices. Molecular biologists used...bromide, less stop buffer and miniscule amounts of plasmid sample. How satisfying? With practice, I got ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...collection Read our molecular biology reference Find plasmids for your research ... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech and is in a freezer... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...compared to transgenic animals (17). These viral plasmids are available through Addgene. The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here. References: H... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...accelerate your research. Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...other scientists save resources by sharing plasmids Find plasmids at Addgene ... -
Year of the Camelid: Antibody Style
TypeBlog Post... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...Membrane Proteins More resources from addgene.org Plasmids encoding nanobodies at Addgene Sybody Generation... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...