We narrowed to 359 results for: Nov
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TypeBlog Post...CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse Geneticist's...
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CRISPR 101: Validating Your Genome Edit
TypeBlog Post...was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015 Nov 5;5:16277. PubMed PMID: 26538064. PubMed Central ... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post..., Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1;450(7166):56-62. PubMed. A technicolour approach... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield." Gene...Link opens in a new window) . Last reviewed on: November 7, 2023... -
Gibson Assembly Protocol
TypeProtocol...colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear ... -
General Transfection
TypeProtocol...limited effect on cell growth. Last reviewed on: November 7, 2023... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...SimplyBlue SafeStain, Thermo Fisher, LC6060 4-12% NuPage Novex bis-tris mini gel, 1mm thick, 10-well, Invitrogen... -
Lentivirus ddPCR Titration
TypeProtocol...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Hum Gene Ther Methods... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection... copGFP from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within...on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide Find...Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and the...Medicine have deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...process as it can be used in blue–white screening . Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses... more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral... -
TALEN Guide
TypeCollection...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide...zinc finger community ( see interview with Adam Bogdanove ), progress on TALe research has been swift. The...Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final...technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas’s group from the University...backbones in just a few steps ( Figure 2 ). The Bogdanove group also hosts web-based software to help scientists...hundreds of unique visitors every month. The Voytas/Bogdanove TALEN kit came online in the summer of 2011 and...Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(... -
Immunology Research Plasmids and Resources
TypeCollection...urokinase receptor CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634...receptor - NODAL nodal homolog (mouse) MGC138230 NOV nephroblastoma overexpressed gene CCN3, IGFBP9 NPFF... in early stage tumors-like 1 cdnf ARTN artemin ENOVIN, EVN, NBN AVP arginine vasopressin ADH, ARVP, AVP-NPII...tissue growth factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (... -
COVID-19 Resources
TypeCollection...Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called...speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like...protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources...coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics:...2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus Information Center The Lancet COVID-...COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access Collections of...2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein . Cathepsin L - Bosch... -
Viral Vector Guides and Plasmids
TypeCollection...lines or clinical use. Adenoviral Vector Guide | Adenoviral Plasmids Adenoviral vectors have high packaging...kb 18–26 nm Dividing/Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb ... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...viruses originally discovered as contaminants of adenovirus stocks. One major advantage of using AAV for ...the presence of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This ...infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding... Helper plasmid for AAV packaging, expressing adenovirus E4, E2A and VA James M. Wilson RepCap Plasmids... -
CRISPR Guide
TypeCollection...Seegers, C. L., Bollen, S., Jore, M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries...control both when and where genome editing happens. CASANOVA , developed by Dominik Niopek’s lab , is a way...It has also been used extensively to screen for novel genes that regulate known phenotypes, including ... maintained by the Jennifer Doudna lab at the Innovative Genomics Institute, University of California,... 35301321 Edraki, A., Mir, A., Ibraheim, R., Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J....30403660 Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg,..., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov, K., Van Der Oost, J., & Zhang, F. (2017). Multiplex... -
Luciferase Plasmid Collection
TypeCollection... of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly... Return to Top Other Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with...and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...Renilla luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated decay reporter... -
Resolute Plasmid Collection
TypeCollection...members from industry and academia financed by the Innovative Medicine Initiative (IMI) of the European Union...RESOLUTE project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under...the European Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: Representation... -
Brain Initiative Collection
TypeCollection...Collection The Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to...brain through the development and application of innovative tools enabling large-scale real-time interrogation...brain generated through this effort will provide novel ways to treat, cure, and even prevent brain disorders...