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We narrowed to 359 results for: Nov

Showing: 41 - 60 of 359 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...ways to circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences... from IGI!) Synthetic S. pyogenes Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed...2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding specificity...remaining variants (VQR, EQR, and VRER) recognize novel PAM sequences (shown above). The VQR, EQR, and VRER... Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub...Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier...

  9. General Transfection

    Type
    Protocol
    ...limited effect on cell growth. Last reviewed on: November 7, 2023...

  10. Gibson Assembly Protocol

    Type
    Protocol
    ...colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear ...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Hum Gene Ther Methods...

  13. Adenovirus Guide

    Type
    Guide
    ... Guides Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral genome necessary... of all adenoviral plasmid components, see the Adenoviral Plasmid Elements table . Adenoviral Generations...Introduction to Adenovirus Adenoviral Vector Production and Troubleshooting Adenoviral Delivery of CRISPR... No more helper adenovirus: production of gutless adenovirus (GLAd) free of adenovirus and replication-competent... Kosai, K. I. (2021). Adenovirus biology, recombinant adenovirus, and adenovirus usage in gene therapy...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...

  14. TALEN Guide

    Type
    Collection
    ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide...zinc finger community ( see interview with Adam Bogdanove ), progress on TALe research has been swift. The...Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final...technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas’s group from the University...backbones in just a few steps ( Figure 2 ). The Bogdanove group also hosts web-based software to help scientists...hundreds of unique visitors every month. The Voytas/Bogdanove TALEN kit came online in the summer of 2011 and...Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Core Lentiviral and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector... and copGFP from the PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within ...Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of...have kindly deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...transformants will instead form white colonies. Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses...

  16. Viral Vectors

    Type
    Collection
    ...their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are commonly used as vaccines ...immunogenic response they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their...preferentially infect and kill cancer cells. Adenoviruses have high transduction efficiency and direct...4.5 Kb 18-26 Dividing/Non-dividing No* Very Low Adenovirus Transient double stranded linear DNA >8 Kb 105...Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide University....2010.12.009. PMID: 21167871 Wold WSM, Toth K. Adenovirus vectors for gene therapy, vaccination and cancer...

  17. COVID-19 Resources

    Type
    Collection
    ...Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called...speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like...protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources...coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics:...2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus Information Center The Lancet COVID-...COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access Collections of...2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein . Cathepsin L - Bosch...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...urokinase receptor CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634...receptor - NODAL nodal homolog (mouse) MGC138230 NOV nephroblastoma overexpressed gene CCN3, IGFBP9 NPFF... in early stage tumors-like 1 cdnf ARTN artemin ENOVIN, EVN, NBN AVP arginine vasopressin ADH, ARVP, AVP-NPII...tissue growth factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...NA E nd A ccumulation during T urnover) : Single-molecule mRNA turnover biosensor that utilizes Renilla... of Luciferase Tools in our Collection Modified/Novel Luciferases AkaLuc : A modified firefly luciferase.... LuxSit-i : An artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase... of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...viruses originally discovered as contaminants of adenovirus stocks. One major advantage of using AAV for ...the presence of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This ...infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding... Helper plasmid for AAV packaging, expressing adenovirus E4, E2A and VA James M. Wilson RepCap Plasmids...
Showing: 41 - 60 of 359 results