We narrowed to 77 results for: codon optimized

Learn about hot new tools in our collection!

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation to directed differentiation.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your gene of interest.

Learn more about E. coli strains used for protein overexpression.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.

Find prime editor plasmids for (search and replace) genome editing.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

Take a look at these 5 great apps to make your lab life easier including tools from Gibco, LabArchives, NEB, ThatsMyStapler, and iGreenEarth.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.