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We narrowed to 71 results for: codon optimized

Showing: 41 - 60 of 71 results

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression...provided in both open (no stop codon) and closed (including a stop codon) formats. The collection is compatible...directly for drug screening or easily shuttle the optimized, expression validated GPCR sequences to any desired...strains remains seriously limited, as prior optimizations are required for each individual target species...being generated, there is a need for genetic optimization of industrial yeast strains. However, industrial... the well-known yeast two-hybrid assay are not optimal for studying native mammalian protein interactions...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...version of Firefly luciferase that has been codon optimized for expression in mammalian systems. Other ... Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory...

  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...less efficient than Cre recombinase. FLPe codon optimization resulted in FLPO, which shows similar efficiency...loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different FLP...first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient in mammalian...identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998)....

  6. Antibody Plasmid Collection

    Type
    Collection
    ...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...which circumvents the problem of in-frame amber codons in scFvs A new expression vector facilitating production...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...talk about western blot optimization and troubleshooting.   Before you can optimize or troubleshoot a western...backgrounds.  Once you’ve optimized antibody concentration, you can optimize ‌incubation conditions to...want to spend optimizing it. Blots for quantification generally require more optimization than blots used...with running a gradient, which will allow you to optimize the concentrations of your various blot reagents... a Western Blot post to make sure you have the optimal gel for your experiment.   Ladders If you need ...trickier than gels and may require multiple runs to optimize or troubleshoot.   Before you start… Mark your... semi-dry are not working, you’ll want to try optimizing your transfer buffer.  Adjusting the buffer For...

  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and ...issues as traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require... emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution is to couple ...

  10. Hot Plasmids - October 2020

    Type
    Blog Post
    ...delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors. The system ...follow these same assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing...destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available for both monocot and... paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for efficient germline and somatic...Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...

  13. Lentiviral Prep Service

    Type
    Collection
    ...Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  17. Open Enzyme Collection

    Type
    Collection
    ... (Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...
Showing: 41 - 60 of 71 results