...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain...targets the receptor to specific DNA sequences. Ligand binding triggers a conformational change in the...

...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...

...MA, 02139 Organizing Committee Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene Organizing Committee... your permission). Materials don't have to be organized (yet), we will take care of that. Note that you...

...Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...

...Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...

...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

... for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...monitor structural transitions in protein self-organization. Wan et al. Cell Rep Methods. 2022 Mar 28. Follow...CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics...

...particular organism, and the library is only compatible with cells derived from that organism. All of our...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...collection of synthetic biology plasmids for use in C. elegans....collection of synthetic biology plasmids for use in C. elegans. Worm Plasmids Search the table by keyword or sort...

...curated collection of plasmids for cancer research, organized by signaling pathway. ...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...

...PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...detailed descriptions of chemogenetic receptors, ligands, and how to use these tools in your experiments...

...and changes of voltage all contribute to assess ligand action/engagement. Protein production is used for...assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase...

...customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols...

...small molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...unresponsive to endogenous ligands, could be activated by synthetic ligands. Specifically, Receptors Activated...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have few, if any... Figure 2: DREADDs, their ligands, and signaling properties. DREADD Ligands Muscarinic-receptor based ...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important... Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et al. 1998). These...

...cell line. Fujita T, Kitaura F, Yuno M, Suzuki Y, Sugano S, Fujii H. DNA Res. 2017 Oct 1;24(5):537-548. ...regions by enChIP-Seq. Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish ...

...selection is not required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally...endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally...

...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...

...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...