We narrowed to 610 results for: gan

...conjugated to an organic fluorophore or other small molecule. By using different ligand-dye conjugates,... to help you plan your experiment. Tables are organized by color of the fluorescent protein. The content...flexibility of self-labeling tags on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag ...

... on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...can be influenced by many factors, such as the organism or cell type, relative position and orientation...FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more...

...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

... the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes and binds ...interest. Inducible: The recombinase may be fused to a ligand-binding domain such as the human estrogen receptor...sequence of the recombinase to align with your organism's preferred codon usage will improve expression...

...Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine...orthogonal pairs of aaRS and tRNA that work for one organism may not work for another. The orthogonal aaRS ...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...

...collection of synthetic biology plasmids for use in C. elegans....collection of synthetic biology plasmids for use in C. elegans. Worm Plasmids Search the table by keyword or sort...

...biosensors. Subcellular Localization Visualize organelles and subcellular structures with well-characterized...infrared probes to study molecular processes in organs and whole animals. Additional Resources at Addgene...

...curated collection of plasmids for cancer research, organized by signaling pathway. ...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...

...additional plasmid containing a Cas enzyme or cells or organisms in which Cas has been integrated into the genome...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...

...PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...detailed descriptions of chemogenetic receptors, ligands, and how to use these tools in your experiments...

...and changes of voltage all contribute to assess ligand action/engagement. Protein production is used for...assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase...

...customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols...

...expression plasmids to study ER contact with other organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically...CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics...

...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than letting the... synthesizing your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry...linked to, say, a chemical dye or other functional ligand, your protein of interest will end up linked to...Just express the fusion protein and add a specific ligand! A self-labeling protein gives you the flexibility...flexibility to choose different colors or ligands for different experiments without having to clone a whole ...structure represents a fluorophore or other useful ligand, gray structure is the protein of interest, and...

...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...some kind of pre-analysis. At the end of 2014, I began developing software to make sgRNA design accessible...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends...

...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...people from various organizations in science and beyond. For example, I help organize scientific workshops...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...the ability to work with others outside one’s organization was found in the aforementioned survey as one... partnerships where we can leverage another organization’s expertise to our mutual benefit.  Our partnership...small: Collaboration opportunities within an organization but outside your comfort zone As part of my ...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish ...

...with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains can... binding ligands with high affinity. This extended-open state is key to strengthening ligand binding, ...almost all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other...often express multiple integrins that bind the same ligand, making it difficult to dissect the function of... antibodies in the collection are able to block ligand binding — and therefore interrupt integrin function...ascertain which integrins are responsible for specific ligand-binding functions, all key to basic biomedical ...intracellular ends and large ectodomains containing ligand-binding heads and long legs that protrude from ...

...variety of recombinase systems. Picking Ligands Picking the right ligand is also important for building a chemogenetic...reduced off target activation by ligands. PSAMs themselves are modified ligand binding domains. When coupled... Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes...a method for targeting receptor expression as a ligand and receptor pair. Targeting Receptor Expression...expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier.  Another...  Another consideration when picking ligands for chemogenetic studies is whether or not your study is aimed...reprogrammed to respond only to inert, nonnative ligands. However, they retain their intracellular interactions...

...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many...peptide of interest and produce that peptide in your organism of choice. Unfortunately, codon preferences make...and expect your sequence to express well in any organism. So what are the evolutionary constraints that... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons... tRNAs with the appropriate amino acids. If an organism uses only a subset of codons, it only needs to...