...genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...

...Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...

...visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...

...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...

...genomic loci at once (get more information on multiplexing gRNAs here). Cas9 is commonly tagged with a ...Zhang Y, Qi Y (2015) A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation...Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your ...

...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...

...those times you need more than one?   Multiplex assays Multiplex assays involve multiple antibodies, each...

...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

... 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning...Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular Cloning...

... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

...screening, barcoding, or other high throughput multiplexed experiments. These pooled plasmids differ by ...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...

...via the links below: ID Plasmid 53370 pSQT1313 (multiplex gRNA expression plasmid) 53369 pSQT1601 (optimized...

...detection (14-20). LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...

...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...