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One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

Learn about the Professional Development Hub's work to support science trainee career transitions. Register for the event covering findings from their report!

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Changes in export control regulations have make rabies and VSV-G plasmids more accessible to researchers around the world.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

Binning Singletons is a networking and mentoring program for conference attendees. Learn about the program and how you can participate as a mentor or mentee.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

Protocol for Ligation Independent Cloning

Protocols for plasmid cloning by PCR.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.