Addgene

We narrowed to 152 results for: yeast

Showing: 41 - 60 of 152 results

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Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

... million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of... circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate two...
Protocol - How to Inoculate a Bacterial Culture

Type

Protocol

... 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has...
Our Most Popular Blog Posts from 2019

Type

Blog Post

...covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher...
Easing the Protein Purification Process with pCri

Type

Blog Post

...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities...
Celebrating Accomplishments in the Lab

Type

Blog Post

... a lab day trip each year and as we do a lot of yeast research, a few of those have been trips to breweries...
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

Type

Blog Post

... expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks...
New CRISPR Web Resources and #12DaysofCRISPR Recap

Type

Blog Post

...plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been ...
Antibodies 101: Affinity Tags

Type

Blog Post

...proteins within the expression system (bacteria, yeast, mammalian, etc.). To individually isolate a protein...
Plasmids 101: Gene, enhancer, and promoter trapping

Type

Blog Post

...historically been used extensively in fly, mouse, and yeast models. Why traps are useful Traditional genetic...
What the HEK?

Type

Blog Post

... with other protein production systems, such as yeast. HEKs can also grow in suspension, which allows ...
Meet the New Addgene Board Members!

Type

Blog Post

...Paul Nurse,” Stegmeier says. “They took a dividing yeast and did a genetic screen to identify the factors...
Plasmids 101: What is a plasmid?

Type

Blog Post

...exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids provide one or more...
Some Like it Hot: Thermostable GeoCas9

Type

Blog Post

...for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some...
Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

...detail selection in non-bacterial cells such as yeast or mammalian cells. Name Class Mode of Action...
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Type

Blog Post

...destabilized GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

..., the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell lines (Nishimura...
Advice for Moving Into Sales After Your Science Postdoc

Type

Blog Post

...with and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they ...
Plasmids 101: Inducible Promoters

Type

Blog Post

..., the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Type

Blog Post

...a transcription activation system co-opted from yeast (1). It is a Drosophila geneticist’s main workhorse...
Plasmids 101: Screens vs. Selections

Type

Blog Post

...assayable with CRISPR. Another method, popularized in yeast, are temperature sensitive screens. These involve...

Showing: 41 - 60 of 152 results

Results per page:

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Protocol - How to Inoculate a Bacterial Culture

Our Most Popular Blog Posts from 2019

Easing the Protein Purification Process with pCri

Celebrating Accomplishments in the Lab

Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

New CRISPR Web Resources and #12DaysofCRISPR Recap

Antibodies 101: Affinity Tags

Plasmids 101: Gene, enhancer, and promoter trapping

What the HEK?

Meet the New Addgene Board Members!

Plasmids 101: What is a plasmid?

Some Like it Hot: Thermostable GeoCas9

Plasmids 101: Antibiotic Resistance Genes

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Advice for Moving Into Sales After Your Science Postdoc

Plasmids 101: Inducible Promoters

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Plasmids 101: Screens vs. Selections

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