We narrowed to 138 results for: yeast
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TypeBlog Post...you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of these resources...
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Which Fluorescent Protein Should I Use?
TypeBlog Post...bacteria studies, whereas GFPS65T is better suited for yeast studies (24-30°C). Brightness: Brightness is a measure... to express the FP in acidic environments (e.g. yeast cytosol, which is slightly acidic, or synaptic vesicles... -
Synthetic Biology - Yeast
TypeCollection... Plasmid Collections Synthetic Biology Yeast Synthetic Biology: Yeast SynBio Resources...collection of synthetic biology plasmids for use in yeast. Yeast Plasmids Search the table by keyword or sort...plasmids for use in yeast. Plasmid... -
CRISPR Plasmids - Yeast
TypeCollection... CRISPR Yeast CRISPR Plasmids: Yeast Activate Base Edit Cut Interfere...have been designed for use in yeast. CRISPR...following CRISPR plasmids have been designed for use in yeast. To find emtpy gRNA plasmids, visit Addgene's Empty...Expression Vectors page and search the table for "Yeast". Activate ID Plasmid Gene/Insert Promoter Selectable... -
Microbiology Resources
TypeCollection... and yeast expression vectors : FX cloning System - Geertsma and Dutzler Labs EasyClone 2.0 Yeast Toolkit...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for ...and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for...-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common...depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The ...Link opens in a new window) National Collection of Yeast Cultures (NCYC) (Link opens in a new window) NIH... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...to create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and ... genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog Post...Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed PMID...applications PA-FPs have been developed for use in yeast, as parts of biosenors, and much more. Do you have... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression PATagRFP 562 595...8NeGFP - Zebrafish/Xenopus/Worm/Sea urchin pKT0128 - Yeast Expression EGFP-pBAD - Bacterial Expression mEGFP...-mBaoJin - Bacterial Expression P415 ymBaojin - Yeast Expression mGreenLantern 503 514 73 5.6 14 min Monomer...- Mammalian AAV Expression pBAD-mGreenLantern - Yeast Expression Clover 505 515 84 6.1 30 min Prone to... Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K) dClover2...Expression mVenus C1 - Mammalian Expression pKT0090 - Yeast Expression mVenus-pBAD - Bacterial Expression SYFP2...Expression mGold 515 530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mGold2s... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of... circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate two... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid... S. pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector... marker selection TRP1 Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible...our Plant Plasmids and Resources collection page Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination ...3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag...eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion...C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector that adds a SV40 NLS Myr Membrane... Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher... -
CRISPR Plasmids and Resources
TypeCollection...designed for use in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed...organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and... -
Easing the Protein Purification Process with pCri
TypeBlog Post...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities... -
Celebrating Accomplishments in the Lab
TypeBlog Post... a lab day trip each year and as we do a lot of yeast research, a few of those have been trips to breweries... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol... 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has... -
CRISPR Plasmids - dCas9-FokI
TypeCollection... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been ... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post... expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks...