We narrowed to 147 results for: yeast

1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   ...to create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and ... genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369...

2. Addgene’s Top 10 Blog Posts from 2014

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   ...Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered...

3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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   ...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed PMID...applications PA-FPs have been developed for use in yeast, as parts of biosenors, and much more. Do you have...

4. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ... million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of... circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate two...

5. Protocol - How to Inoculate a Bacterial Culture

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   Protocol

   ... 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has...

6. Our Most Popular Blog Posts from 2019

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   ...covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher...

7. Easing the Protein Purification Process with pCri

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   ...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities...

8. Celebrating Accomplishments in the Lab

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   ... a lab day trip each year and as we do a lot of yeast research, a few of those have been trips to breweries...

9. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been ...

10. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    ... expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks...

11. Antibodies 101: Affinity Tags

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    Blog Post

    ...proteins within the expression system (bacteria, yeast, mammalian, etc.). To individually isolate a protein...

12. Meet the New Addgene Board Members!

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    ...Paul Nurse,” Stegmeier says. “They took a dividing yeast and did a genetic screen to identify the factors...

13. Plasmids 101: What is a plasmid?

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    ...exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids provide one or more...

14. Plasmids 101: Gene, enhancer, and promoter trapping

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    ...historically been used extensively in fly, mouse, and yeast models. Why traps are useful Traditional genetic...

15. What the HEK?

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    ... with other protein production systems, such as yeast. HEKs can also grow in suspension, which allows ...

16. Some Like it Hot: Thermostable GeoCas9

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    ...for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some...

17. Plasmids 101: Antibiotic Resistance Genes

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    ...detail selection in non-bacterial cells such as yeast or mammalian cells.   Name Class Mode of Action...

18. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...destabilized GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin...

19. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ..., the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell lines (Nishimura...

20. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post

    ...with and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they ...