We narrowed to 893 results for: plasmid
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TypeBlog Post... Source Lab Resources on Addgene.org Try the Plasmid Land Game ...
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General Transfection
TypeProtocol...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ... -
Working 9-5: an Addgene Co-op
TypeBlog Post... the submitted sequences were a concern to the plasmid function. The steps of the training process gave... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology Protocols ... -
Pouring LB Agar Plates
TypeProtocol...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies... -
A History of Genome Engineering in Popular Culture
TypeBlog Post... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ... -
Finding Your Perfect Job After University
TypeBlog Post...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...neurons that could be activated with light. Find plasmids from the Isacoff lab! They then tested what kind... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...writing about new scientific techniques and cool plasmids. Creating graphics were usually the last thing... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...production. Whether redesigning viral packaging plasmids, investigating different viral production strategies... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...Check out CRISPR Topic Page Find CRISPR Nickase Plasmids Browse the CRISPR Guide ... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post... it easy for scientists to deposit and request plasmids (via web interface, processes, distribution, and... -
Antibodies 101: Epitope Availability
TypeBlog Post...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... -
New Viral Vectors - Winter 2025
TypeBlog Post...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to... -
Antibodies 101: Affinity Tags
TypeBlog Post...Antibody protocols Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...resources on Addgene.org CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development ...