Transfection for Recombinant Antibodies
Type
Protocol
...Recombinant Antibodies You may also like... Antibody Plasmid Collection Antibodies Blog Posts Antibody Guide...suspension HEK293 cells with recombinant antibody plasmids using Polyethylenimine Max as a transfection reagent...General Considerations For antibody-expressing plasmids containing the SV40 origin of replication use ... SV40 large T antigen. For antibody-expressing plasmids containing the Epstein-Barr virus origin of replication...meter Reagents HEK293 cells Recombinant antibody plasmid DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge... Transfer the PEI-MAX and recombinant antibody plasmid DNA sample to the biosafety cabinet and incubate...biosafety cabinet. Add 180 µg of recombinant antibody plasmid DNA to one tube of 6 mL BCD TFX. Cap the tube and...