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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 899 results for: plasmid

Showing: 581 - 600 of 899 results

  3. General Transfection

    Type
    Protocol
    ...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  6. Science as Art

    Type
    Blog Post
    ... Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... the submitted sequences were a concern to the plasmid function. The steps of the training process gave...

  9. Pouring LB Agar Plates

    Type
    Protocol
    ...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...

  11. Finding Your Perfect Job After University

    Type
    Blog Post
    ...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

  20. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to...
Showing: 581 - 600 of 899 results