We narrowed to 918 results for: plasmid

Learn what a high fidelity DNA ligase is and when you might need one.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Learn about the most common injection routes in mice, including their key differences and uses.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

Quickly isolate mammalian cells clones using the agarose cloning method.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Congratulations to Addgene depositor David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper!

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

Announcing Addgene's AAV Data Hub! The Data Hub is an open platform for scientists using AAV to share practical information about their experiences.

Addgene has many videos to walk you through lab protocols. Find videos about AAV purification, AAV titration, and the basics working with tissue culture.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Get an inside look into the software development process. at Addgene.

Learn ways you can bring your science to elementary and middle school by hosting events and giving talks geared towards elementary and middle school students.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Find tips on how to write your first review article - a useful experience for any scientist.