We narrowed to 913 results for: plasmid

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

Quickly isolate mammalian cells clones using the agarose cloning method.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Congratulations to Addgene depositor David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper!

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

Announcing Addgene's AAV Data Hub! The Data Hub is an open platform for scientists using AAV to share practical information about their experiences.

Addgene has many videos to walk you through lab protocols. Find videos about AAV purification, AAV titration, and the basics working with tissue culture.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Get an inside look into the software development process. at Addgene.

Learn ways you can bring your science to elementary and middle school by hosting events and giving talks geared towards elementary and middle school students.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Find tips on how to write your first review article - a useful experience for any scientist.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.