We narrowed to 896 results for: plasmid
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TypeBlog Post... Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...
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Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post... Blog Learn About Our Cancer Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...with the post-design process such as primer and plasmid design. Examples: “multiplex design”, “multi-method... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush... -
Protocol - How to Run an Agarose Gel
TypeProtocol...Electrophoresis You may also like... Restriction Digest of Plasmid DNA Purifying DNA from an Agarose Gel DNA Ligation... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...Recombinant Antibodies You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection... -
Western Blot
TypeProtocol...Blot Western Blot You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...Protein A or Protein G You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...Indirect ELISA Method You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibodies 101: ... -
Lentivirus ddPCR Titration
TypeProtocol...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies ... -
Deep Dive: qPCR
TypeBlog Post...Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR... -
Molecular Biology Reference
TypeGuide...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...guide on molecular cloning techniques . Plasmid Elements Plasmids used by scientists today come in many ... -
Lentiviral Vector Guide
TypeGuide...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...rev ; separated into two plasmids in third generation systems Envelope plasmid — contains packaging gene...