We narrowed to 913 results for: plasmid

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Addgene's Advisory Board helps steer and promote Addgene in its goal of sharing science. We welcome CRISPR expert, John Doench, to the Advisory Board.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

We share our principles of clear and accessible writing and show how to apply them through the development of alt text.

Tim Miles, the guest speaker at our recent AAV webinar, and our webinar panel of experts, is finishing up their Q&A session.

Learn about SF-iGluSnFr, an updated glutamate biosensor from Loren Looger's lab.

A quick overview of antibody storage, buffers, and conjugates.

An overview of secondary antibodies and the indirect approach in immunoimaging assays.

An antibody’s isotype is determined by its heavy-chain constant region and influences what role the antibody plays in an immune response.

Opto-nanobodies (OptoNBs) are nanobodies that can be controlled by light. Learn how you can use OptoNBs to purify proteins or to modulate cell signaling.

Learn how you can use new photosensitizers to specifically kill target cells.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Curious about conjugating your antibody? Read our 101 on antibody conjugation chemistry!

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Rosella is a fluorescent biosensor that you can use to study autophagy.

What is a career coach and how can scientists find the right one for them?