We narrowed to 899 results for: plasmid

...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...fluorophore) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR...

...Selected Download Select Plasmids 101 September 2023 | Fourth Edition About Plasmids 101 September 2023 ...popular Plasmids 101 blog series, this eBook covers everything you need to know about plasmids, from parts...

...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...

...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...about various scientific concepts related to our plasmid collection. Find links to the guides below. Antibodies...

...mainly uses next-generation sequencing (NGS) for plasmid verification, Addgene has used a number of primers...useful in your sequencing reaction, find your plasmid’s page and see what primers are listed under "5'...questions about choosing the best primer for your plasmid? Email us at [email protected] . For additional ...additional information on molecular biology, plasmids, and recombinant DNA, please consult Addgene's Molecular ...GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids Forward pCasper-F GGGTTTTATTAACTTACAT 5' end of...

...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...

...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity....learn more, or explore Addgene's Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics... ready-to-use AAV preparations of chemogenetic plasmids . Additionally, genetically engineered animal ...available. Table 4: Common promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons...on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted to specific tissues, cell...

...Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids Adeno-associated...researchers need to use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene or...and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging plasmids, or RepCap ...Available AAV Packaging Plasmids This table lists all packaging plasmids (Rep/Cap plasmids) in Addgene’s repository... Viral Plasmids Adeno-Associated Viral (AAV) Plasmids Adeno-Associated...interest to introduce in cells. A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep)...encoded in the packaging plasmid will determine the AAV vector serotype. A helper plasmid containing adenoviral...

...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...particles have been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or ...flanked by LTRs Packaging plasmid — contains packaging genes Envelope plasmid — contains packaging gene...Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection...

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common...(a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA...flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation...second-generation, two plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights...Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...

... Viral Plasmids Adenoviral Plasmids Adenoviral Plasmids Shuttle Plasmids Adenoviral Plasmids Adenoviral... generally need to use two plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene ...Browse shuttle plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production...for recombination with the adenoviral plasmid. An adenoviral plasmid containing the rest of the adenoviral...with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can be used...Adenoviral Vector Guide Shuttle Plasmids Browse commonly used shuttle plasmids for adenoviral vector production...production. Want a plasmid with your gene of interest? Search our database for shuttle plasmids with inserts...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 169,261 plasmids . We've...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael... Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits... promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Haloferax sp. Methanococcus... sp. Yersinia pestis Plasmids for Fungi Aspergillus sp. Cryptococcus sp. Plasmids for Protozoa Entamoeba...West Nile virus (WNV) Zika Plasmids for Cyanobacteria Cyanobacteria Plasmids for Prions Prions Don’t see... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...Edit ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/...Epigenetics ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert... ID Plasmid Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene...Editing ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...

...Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors ... Plasmid Collections Depositor Plasmid Collections Depositor Collections...Addgene's repository contains 169,261 plasmids, which includes plasmid collections from depositors. To help... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Recombinant mAb Plasmid Collection Disease Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative...Collection Rett Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization... Science Plasmid Collection E11 Bio PRISM Collection New England BioLabs Cell-Imaging Plasmid Collection...

... Plasmid Collections Plant Plasmids Plant Plasmids and Resources Cloning & SynBio...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...use in plants. The tables below highlight plasmids and plasmid kits spanning a wide variety of molecular.... ID Plasmid Description Promoter Cloning Method PI Synthetic Biology Kits The following plasmid kits ...full selection of plasmids expressing different functional variants of Cas9. ID Plasmid Description PI Genome... Editing Kits The following plasmid kits and collections contain plasmids designed for genome editing .... It also includes plasmids encoding silencing suppressors, such as p19. ID Plasmid Description PI Additional...

...-only plasmids are not limited to a specific CRISPR function, but require an additional plasmid containing...catalog for control gRNAs . All-in-one Plasmids (gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker...Marker PI Publication Return to top gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI...Publication Return to top Multiplex gRNA Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI... Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA plasmids, including: Multiplex...of other CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid....ΔA.Δserb.Amp Plasmid 68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1...pSerOTS-C1* (V70) (Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important...-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

...following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids, visit Addgene's...Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI... top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert...top Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in bacteria. CRISPR...