We narrowed to 957 results for: tes

... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

...into transformed and non-transformed cells yielded rates of targeted mutagenesis similar to those achieved...tools can also be used to deliver Cas9 and DNA templates in somatic cells of adult animals in order to ...

...eliminate every cancerous cell. Even so, to pose the greatest threat, cancerous cells still need to successfully...the research community has access to all of the latest resources? In response to this challenge, Addgene...

...The FP is confined to a plane and generally only rotates in 360°, increasing the probability that two FPs... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...

...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

...pathogens to their hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre ...of terminators than are commonly used as we have tested them to show that they work well in plants and ...

...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...

...Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol Streaking...Bacteria on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol...CRISPR Protocol Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic...

...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

...study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career...

...Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

...within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible...

...longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!    How to... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should be...

... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....guide RNA / shRNA inserts Resistance Marker: This indicates the selectable marker used in your model system...

...checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...

...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ...

...of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette...

...transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832...

...for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for...