Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 963 results for: tes

Showing: 741 - 760 of 963 results

  2. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than...-log of the expected titer of the samples being tested. Always include a No Template Control (NTC), i....small aliquot of each standard (enough for 1 or 2 plates) and store at -20 °C. Once a standard is thawed...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...

  3. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation. Shown...Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL... the plate at room temperature for at least 30 minutes with the lid on to give the antibiotic time to ... with smaller size than the 1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...also enabled imaging of their propagation along dendrites for the first time (Figure 3). The team also used...a patient with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  6. PhD Applications After COVID

    Type
    Blog Post
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your technical presentation...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

  9. Transferable Skills: Negotiation

    Type
    Blog Post
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...

  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

  15. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...

  16. Video Library

    Type
    Protocol
    ...Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol Streaking...Bacteria on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol...CRISPR Protocol Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  20. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career...
Showing: 741 - 760 of 963 results