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We narrowed to 162 results for: Cre

Showing: 61 - 80 of 162 results
  1. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ...open-reading frame (DIO). Upon coexpression with cre recombinase (either from a mouse line or viral co-injection...type specificity dependent on the promoter of the cre recombinase while still taking advantage of the robust...an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer required...tracing with fluorescent proteins in nontransgenic and cre driver mice. Current protocols in neuroscience / ...: PMC4242517.  7. Madisen L, et al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced...
  2. Using AAV for Neuronal Tracing

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    Blog Post
    ...often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre in only...labelling the subset of infected cells that also express Cre. Another advantage of AAV vectors is that there are... traced neurons with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection into a conditional...conditional mutant mouse brain that expressed Cre recombinase only in pyramidal cells of the hippocampal...PMID: 4107258. Lo, L., and Anderson, D.J. (2011). A Cre-dependent, anterograde transsynaptic viral tracer...
  3. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ... short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated recombination to turn on or off the expression...a dual-color RNAi “sensor” mouse strain in which Cre expression causes the induction of both DsRed fluorescence...Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and... recombination events at the genetic level. Upon Cre/loxP recombination, each transgene expresses one ...Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems...SUMO tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids... all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow...
  4. Hot Plasmids - August 2020

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    Blog Post
    ...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that...Find this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA...
  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ... the expression of a Cre recombinase contained within the knock-in. Including Cre-mediated marker excision...kilobases of additional sequence being introduced. Cre-mediated excision of the selective cassette minimizes...experimental manipulation. In some cases, injection of Cre is required, however the development of the self-...
  6. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...recombinase that can be used in combination with Cre for intersectional control of specific neuronal populations... We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...
  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...CAG-FLEX: CAG promoter, FLEXed for expression in Cre-expressing cells hSynap: Human Synapsin-1 promoter...hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter...
  8. Hot Plasmids: Fall 2025

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    Blog Post
    ...Once the recipient cells take up the Cre-tagged mitochondria, Cre is released, translocates to the nuclease...mitochondria (Hoover et al., 2025). MitoTRACER relies on a Cre-lox switch to turn recipient cells from red to green...
  9. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette. While this method...mutants [7], it is generally not as efficient as Cre recombinase. However, the use of negative selection...likely due to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific, and has ...
  10. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ... be achieved using specialized promoters, Flp or Cre dependent gene switches, and serotype selection. ...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression...
  11. Hot Plasmids - October 2020

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    Blog Post
    ...targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration...preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory channelrhodopsin...
  12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ...useful tool for marking and tracking cells is the the Cre/lox recombination system. A researcher can flank ...expression is turned on or off in cells expressing Cre. The result of a system like this is a mosaic of ...respectively. The permanent labelling of cells using cre-lox is advantageous when compared to photoactivable...
  13. Hot Plasmids: Summer 2025

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    Blog Post
    ...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene...synapses. In addition, ATLAS itself could be expressed Cre-dependently to allow tracing exclusively from genetically...
  14. Hot Plasmids Spring 2024

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    Blog Post
    ... Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent AAV1 vectors and are suitable for extended...found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled ...
  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post
    ...(Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the endogenous MECP2 gene...excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely...
  16. Delivery Methods for Generating iPSCs

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    Blog Post
    ...formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses address some...considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is the same...standard lentiviral vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes...
Showing: 61 - 80 of 162 results