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...development of new applications. The first CRISPR papers described two main categories of genome edits. ... lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less off-target activity...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based on shared...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that... PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve the tool...

...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable Markers...collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources collection page...Additionally, tags are commonly added to aid in protein purification. Just remember to remove the stop codon for ...myristoylation signal GST Protein purification pEBG - N-terminal GST for mammalian...expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial expression with MBP-TEV...MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression vector for His8...

... CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CAT catalase MGC138422...hCD40L CD70 CD70 molecule CD27L, CD27LG, TNFSF7 CECR1 cat eye syndrome chromosome region, candidate 1 ADGF,...CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL, CTSL, FLJ31037,...phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide...and adaptive immune signaling and cell-cell communication. Plasmid...

... PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker Publication Return... PI Publication Return to top RNA Editing ID Plasmid Gene/Insert Selectable Marker PI Publication Return...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert... ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Visualize ID Plasmid Gene/Insert...

...plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes necessary for the...the AAV genome, but are required for AAV viral replication. This collection provides a curated selection...production suitable for a variety of serotypes and applications. Read our AAV vector guide for more information...plasmids, or RepCap plasmids, encode the AAV replication and capsid proteins necessary for AAV vector ...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...

...for protein purification. The pTD Plasmid Series contains plasmids suitable for replication in many gram-negative...expression plasmids that can be used for protein purification, protein visualization, study of protein interactions...Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...expression plasmids to find out more! Protein Purification To study the function, structure, and activity...through affinity chromatography. To facilitate the purification process, proteins are often expressed alongside...Browse our most popular plasmids for protein purification. Commonly used tags, cleavage sites, and signal...

...make targeted epigenetic modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic...designed for epigenetic modification. To learn more about epigenetic modification with CRISPR and other ...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...removal of H3K4 and H3K9 methylation Epigenetic modification is typically targeted to a specific promoter...or enhancer for your gene of interest. These modifications persist over time and are potentially heritable...chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...

...Achieving your desired edit comes down to location, location, location. Positioning your chosen Cas enzyme ...designing the gRNA component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations...the table below, we highlight the most common categories of genetic manipulations used in mammalian cells...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...start sites that could allow for expression of a truncated protein. Targeting an exon coding for an essential... Select a cut site as close as possible to the location of the desired edit, ideally less than 10 bp away...complex. You then delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach...

...target sites, and catalyzes recombination between them. Most SSR enzymes are categorized into two distinct...a genomic attP site. So far, their practical application has been limited by low integration efficiency...after recombination (inversion, deletion, or translocation) depends on the orientation of each target site...are on separate DNA molecules (in trans), a translocation event is generated between the target sites....(Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer...By expressing the enzyme at specific times or locations using cell-specific promoters or inducible systems... used for more than inversion, deletion, or translocation of a gene of interest. Using Cre or Flp as examples...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ID Plasmid Gene/Insert...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken Beta actin...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI Publication Return to top Cut...Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication Return to...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...top Purify ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO...Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding... to top RNA Targeting ID Plasmid Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...the material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1....the material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1....sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample is denoted...denoted with a .T suffix (for example, AAV1.T). The catalog ID for the 100 µL sized sample does not have the...from the CAG promoter. For information about each catalog item, including the titer and quality control, ... synpasin promoter. For information about each catalog item, including the titer and quality control, ...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

...Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato...Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-...Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10...Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B22...Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to...Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...all the antibodies currently available from our catalog. Addgene values transparency and accessibility ...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...intractable via animal immunization. Addgene's antibody catalog is a community resource that is growing! We welcome...

...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed...2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under ...

...Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Selectable Marker PI Publication Return to...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

... PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Description Gene...Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...