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Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling and cell-cell communication.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Get tips on writing your next successful grant.

Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's Disease, and others.

Addgene participated in the 2015 MassBioEd career exploration day to teach students some of the great careers available in biology.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Get advice on story telling and science communictaion from Nature Jobs Editor, Jack Leeming.

We discuss some of the many events organized by Science in the News as well as how you can easily get your own science communication student group started.

In this blog post, learn about a new FRET-based biosensor for studying epigenetic modifications and the roles they play in inheritance/disease.

Stephanie Hays provides a breakdown of why people all over the world are planning to March for Science.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

We sit down with SciComm experts, Vini and Amy, from the graduate student group Science in the News.

Learn what it's like to be a Senior Editor at Science Magazine from Pamela J. Hines PhD.

Learn how Addgene uses SnapGene to aid in plasmid quality control and check out some tools you might find useful for your own plasmid verification processes.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

Find tips on how to perform both large and small scale plasmid verification and sequencing.