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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 101 results for: donor

Showing: 61 - 80 of 101 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... kb. This system is transfected into cells as a donor plasmid containing the transposon, along with a ...helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest flanked...

  5. Promega Plasmid Collection

    Type
    Collection
    ... assays use NanoLuc Luciferase as a BRET energy donor and HaloTag protein, labeled with the NanoBRET 618...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ... efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities...

  14. Cloning

    Type
    Guide
    ...This fragment can then be cloned into a gateway donor plasmid which contains compatible attP sites via...Now that your DNA fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible...can clone your gene of interest one time into a donor plasmid (or acquire one that already has your gene...allowing for rapid cloning of PCR products into donor plasmids without the need for restriction enzyme...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    ... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...

  18. CRISPR Guide

    Type
    Guide
    ...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...recombinase or phage derived serine integrases, insert donor DNA at “landing sites” defined by relatively short...
Showing: 61 - 80 of 101 results