We narrowed to 93 results for: multi

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Learn how to design a CRISPR screening experiment targeting enhancers.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your gene of interest.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters.