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We narrowed to 575 results for: multi

Showing: 61 - 80 of 575 results

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...that bacteria usually cannot produce functional multi-domain mammalian proteins as bacterial cells are... expression of mammalian proteins that require multiple post-translation modifications for proper protein...

  4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ... is straightforward. Instructors first select a multi-gene pathway to study and a reference organism where...occurs through a well-characterized pathway with multiple enzymatic steps (5). In their experiments, students...Find the Plasmids Here Plasmid features enable multiple student experiments We used TOPO cloning to insert...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    .... A high affinity terminator may be desired for multi-cistronic constructs where high termination efficiency...Polyadenylation is the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger...PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive...

  6. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...forward genetic screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR...any experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen...strengthen your results. Enrichment or depletion of multiple gRNAs targeting the same gene can be strong evidence...

  7. Antibody Guide

    Type
    Collection
    ...five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers. ...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...allows for the amplification of signals by having multiple secondary antibodies binding to a primary antibody..., which is typically the number of antibodies multiplied by two, as each antibody has two binding sites...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...conformation. Because many aaRSs exist within a multi-tRNA synthetase complex, the team investigated whether...SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the...which required protein compartments to co-localize multiple enzymes in a reaction pathway, the MPyV system...

  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...Barbara, et al. "Gene optimization mechanisms: A multi‐gene study reveals a high success rate of full‐length...codons unaccounted for. The result is redundancy; multiple codons encode single amino acids. Evolutionary...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...cells expressing both dCas9 and gRNAs. The multi-gRNA piggyBac vector integrates into genomic DNA...https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi and CRISPRa system for human pluripotent...uniform and sustained expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator... transcript and protein levels. Plasmids for multiplexed piggyBac gRNA delivery and the all-in-one dCas9...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...single-component nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...adjusting the thickness of the laser sheet and using multi-view imaging can provide enhanced resolution (8,...combined light sheet microscopy with sequential multiple-view reconstruction (5). In order to image large...light scattering and absorption.  Finally, the photomultipliers used to detect light in confocal microscopes...acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...through Addgene.  The combination of LSFM with multiple tissue clearing strategies and neuronal labeling...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...savings, and 3) ability to perform single site or multi-site saturation mutagenesis with the same protocol...mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest...a selection system. You can test the effect of multiple mutations in combination and possibly discover...that tile across a region of interest, generating multiple mutants in one PCR reaction. Below is a step-by-step...randomized bases (NNN) at a given codon site and multiple primer sets tile across the protein or region ...where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite orientations...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...containing the sgRNA target sequence can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330...-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports... Nano-Lanterns  Suzuki, K., et al. (5) imaging multiple proteins at the same time in live cells using ..., to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine...the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the dynamics of subcellular... of bright luminescent protein for real-time multicolour bioimaging. Nature Comm. 7, 13718. PubMed PMID...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immunotherapeutic target is never the answer to the multi-level barricades put up by cancer. As mentioned ... use the blood stream to commute to and invade multiple organs, and most importantly escape the immune...therapies must be explored. When attacking cancer, multiple immunotherapy targets can be utilized at the same...
Showing: 61 - 80 of 575 results