Viral Vectors 101: Producing Your rAAV
Type
Blog Post
...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...
We narrowed to 967 results for: Cre
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Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost... -
Of Myc and Men
TypeBlog Post...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https... -
Academic vs. Industry Postdocs
TypeBlog Post...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...starting vector that the depositing lab modified to create their new plasmid. The Addgene Vector Database... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...improvements and adaptations that can provide an increased understanding of DNA binding and gene regulation... -
AAVs in Retinal Gene Therapy
TypeBlog Post...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy!... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...appears to return to normal. The model fit these two discrete phases with an estimate of 62.3% for the COVID... -
An Introduction to Adenovirus
TypeBlog Post...makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early... -
Getting to Know Expansion Microscopy
TypeBlog Post...don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...’t support reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory ... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...dLwaCas13a as a programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for... -
Career Insights: Technical Support Specialist
TypeBlog Post...match your expertise with that of the company. To increase your chances at successfully applying for a position... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post... a singleton), and is proudly "too weird to be credible." Joe is the founder and President of Binning ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...using Chr2 stimulation should result in robust increases in c-fos expression in the targeted brain region... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...ELN team is. Once you’ve made your list of ELNs, create some free or demo accounts and schedule online ...