We narrowed to 1,052 results for: sta

1. Build Your CRISPR Vocabulary

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   Blog Post

   ...Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

2. Year of the Camelid: Antibody Style

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   Blog Post

   ... early years of sdAbs development, the process started with immunizing a camelid (usually llamas or alpacas...

3. Antibodies 101: Affinity Tags

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   Blog Post

   ... suited for their primary application. Ready to start tagging? We are here to help with vectors, protocols...

4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   Blog Post

   ...built-in primer design tools, but here are a few stand-alone sites for general primer design, as well as...

5. Hot Plasmids and Viral Preps - July 2021

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   Blog Post

   ...vitro and have emerged as a powerful model for understanding human physiology, development, and disease. ...

6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   Blog Post

   ...data by biomedical researchers and ready access to stably archived large-scale datasets by computational ...

7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   Blog Post

   ...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

8. Choosing a Good Mentor for Scientists

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   Blog Post

   ...proficiency, communicate your work, maintain ethical standards and interact professionally with other scientists...

9. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   Blog Post

   ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post

    ... much-needed community resource. Instead of painstakingly searching for experimental details in research...

11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

    ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

12. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

13. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    Blog Post

    ...references and web links from my first post and start designing your first fly experiment! Many thanks...

14. New and Upcoming Viral Vectors - September 2019

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    Blog Post

    ...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...

16. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

17. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

18. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

19. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...