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We narrowed to 223 results for: antibody

Showing: 81 - 100 of 223 results

  1. Hot Plasmids - October 2022

    Type
    Blog Post
    ...-Myc antibody here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific...months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository through our...something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron...Waldron The majority of Addgene's more than 50 antibodies target neuroscience-related proteins, but we ...we recently added several broadly useful antibodies for detecting protein tags.  And when you think of ...

  2. Hot Plasmids - January 2023

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    Blog Post
    ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository through our...smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's what... Car constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting...-GFP [N86/38.1R] was among the first batch of antibodies we produced at Addgene and, as you might suspect...targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated against full length...recognize different epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post
    ... targets, similar to how antibodies do. The structure of the common antibody is heftier (~150 kDa) and...fragment derived from a camelid antibody called a heavy chain antibody (HCab). Because of their small ...over several days and isolated special camelid antibodies that bind to the spike proteins in cell culture...especially challenging when trying to develop antibodies against unstable targets like membrane proteins...and they can target epitopes that are hard for antibodies to get to, like ones that are hidden in the molecular... surface. Figure 1: Comparison of common antibody, HCab, and nanobody. For more information read...sybodies against membrane proteins Developing antibodies against proteins embedded in membranes is tricky...

  4. Hot Plasmids: Fall 2024

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    Blog Post
    ...months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...this post: Anti-V5 and other new epitope tag antibodies from IPI New tool for structural studies of E3... reporter of mechanical forces   Epitope tag antibody collection grows By Ashley Waldron We are excited...excited to highlight the Anti-V5 tag antibody produced by our partners at the Institute for Protein Innovation...IPI), part of a new collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1...version of the original V5 tag mouse monoclonal antibody, which recognizes a short, linear epitope derived... proteins) and performs well in many different antibody-based applications.  IPI’s recombinant version...

  5. Hot Plasmids Spring 2024

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    Blog Post
    ...Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated and characterized... for details on the original antibodies. Find Anti-SARS-CoV-2 Antibodies here! Terry, J.S., et al. (2021...months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our...pooled CRISPR screens Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector...10.1038/s41586-024-07259-6. PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley...Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene repository of tools ...tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] and Anti-SARS-CoV...

  6. Twelve Amazing Guest Blog Contributors!

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    Blog Post
    ...Biddle, of Only Good Antibodies, who shared their antibody validation expertise. Antibody Validation for Flow... Caitlin Faulds, who introduced the very first antibody deposit (the Integrin collection) from the Institute...Institute for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who ...

  7. Neurodegeneration Research Collection

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    Collection
    .... See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including ... AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative...including monoclonal antibodies, scFvs, and nanobodies. New and Noteworthy: Browse our growing collection of general... general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3...2023 Apr 10. Explore the recombinant monoclonal antibodies and scFvs for neuroscience research created with...curates information on useful resources such as: Antibodies (Link opens in a new window) Brain Banks (Link...community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post
    ... cytometer and see one of two results for your antibody of interest: two clear populations or a huge smear...post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that for the flow...cleaner readout. A yes-no plot If you are using an antibody to mark the presence or absence of a protein, ...Figure 2: (A) a smeary dot plot showing a range of antibody readouts. (B) A gate on the smeary dot plot shows...negative in your dot plot, looking at a histogram vs antibody plot would be better. The histogram shows peaks...   Figure 4: A histogram plot for a smeary antibody readout. The orange peis the baseline expression...School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional vs Spectral ...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    ...months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository through our...history with protein ticker tape Teaching an old antibody new tricks   New compact, efficient prime editors...4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface, expanding...10.1101/2021.10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While IHC protocols...-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution...] under the Application section of the Addgene antibody page, and learn more about the whole-brain reference... atlas efforts, including a list of validated antibodies, on their webpage: mAb3D Atlas.    Figure...

  10. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different antibody-fluorophore...a conjugated antibody to detect CD45, and I'll need to choose the fluorophore my antibody is conjugated...the Addgene blog Antibodies 101: Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...Cytometry Antibodies 101: Flow Compensation Resources on Addgene.org Addgene's Antibody Guide Ready-to-use ...Ready-to-use antibodies in the Addgene repository Antibody Protocols ...distinguishing live from dead cells. The combination of antibody-fluorophores, fluorescent reporters, and/or dye...reporters, such as GFP, and fluorophore-conjugated antibodies together in the same color panel. In this case...

  11. Hot Plasmids: Spring 2025

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    Blog Post
    ...view from the antibody pages or in the Addgene Antibody Data Hub. Perhaps these antibodies are just the...months we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic...Puromycin-resistant cells were fixed and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084...Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131) or Goat...anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012), and ...counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron Glypicans...

  12. Distribution to Industry

    Type
    Collection
    ...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering and MTA Information Featured Collections...plasmids in our collection! These monoclonal antibodies are produced in-house and undergo application-specific...trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don't See...

  13. Visualizing Protein Turnover In Situ

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    Blog Post
    ...are added to a cellular sample. Both antibodies have a short antibody specific DNA strand added to them....PLA where one antibody was directed toward a protein of interest but the second antibody was directed ...most common approach. With a good and specific antibody and the proper microscope setup we can observe...interactions in situ. In the standard PLA assay, primary antibodies directed towards two endogenous proteins of interests...amplification could only take place if the two antibodies recognized an endogenous protein of interest ...

  14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post
    ...The FLAG antibody has been well characterized and the system allows you to add this same antibody to any...established method called ChIP-seq. ChIP-seq relies on antibodies to recognize and bind transcription factors directly... for transcription factors known to have good antibodies. The results look almost identical. Addgene: ...of the proteins inside a cell have a commercial antibody against them. There’s also a question about how... solved in this CRISPR system with a universal antibody. It’s an epitope not found in human or other cells... So many groups have been struggling with this antibody problem. We thought it was the right thing to ...

  15. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies  ... is a cell, but it could be debris or unbound antibodies.) Each event read by the machine is plotted as...using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/or cellular...  In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to... three different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    ...months we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing for primary...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ... PMID: 37291094.   Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we ...know that we distribute ready-to-use recombinant antibodies as well? Our collection includes a mouse and ...original mouse Fab region with rabbit Fc) anti-FLAG antibody, both of which are also available in trial sizes...sizes. Addgene recommends these recombinant antibodies for use in western blots, though the original parent...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    ...library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide, and...ready-to-use recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform...use the same principles to provide recombinant antibodies, another plasmid-based product that, with Addgene... and two antibody animations.    A blast from the past: Cofounder and CSO Melina Fan setting up...

  18. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies against...months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized ...6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human genes and are recommended...other researchers by contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry... with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results show...CC-BY 4.0 license. Find more neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10...

  19. Hot Plasmids: Summer 2024

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    Blog Post
    ...s Neuroscience Antibody Collection to find a recombinant anti-GFAP [N206A/8R] antibody shared by James...months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our...CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample...doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system ...tissue is identifying the cells you are studying. Antibodies can help reveal cellular identities by targeting...James Trimmer’s lab (Figure 5) as well as antibodies for identifying other cell types in the nervous system...system!  Find recombinant anti-GFAP [N206A/8R] antibody here!   Figure 5: Free-floating rat brain...

  20. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...excited to launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll...world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247 plasmids. We... our distributor partners, with the launch of antibodies in China and expansion of viral services available... in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first IPI collection...
Showing: 81 - 100 of 223 results